Proceedings Paper
Surface-enhanced Raman scattering (SERS) imaging of alkyne-tagged small molecule drug in live cells with endocytosed gold nanoparticles| Format | Member Price | Non-Member Price |
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Paper Abstract
We propose the combination of alkyne-tag and surface-enhanced Raman scattering (SERS) spectroscopy to perform
highly-sensitive and selective drug imaging in live cells. Gold nanoparticles are introduced in lysosomes through
endocytosis as SERS agents, and the alkyne-tagged drugs are subsequently administered in cells. Raman microscopic
observation reveals the arrival of drug in lysosome through enhanced Raman signal of alkyne. Since the peak of alkyne
appears in Raman-silent region of biomolecules, selective detection of drugs is possible without background signal of
endogenous molecules. From endocytosed gold nanoparticles in living HeLa cells, we observed distinct Raman signal
from alkyne-tagged inhibitor of lysosomal enzyme.
Paper Details
Date Published: 9 February 2017
PDF: 6 pages
Proc. SPIE 10046, Visualizing and Quantifying Drug Distribution in Tissue, 1004606 (9 February 2017); doi: 10.1117/12.2256126
Published in SPIE Proceedings Vol. 10046:
Visualizing and Quantifying Drug Distribution in Tissue
Kin Foong Chan; Conor L. Evans, Editor(s)
PDF: 6 pages
Proc. SPIE 10046, Visualizing and Quantifying Drug Distribution in Tissue, 1004606 (9 February 2017); doi: 10.1117/12.2256126
Show Author Affiliations
Jun Ando, Osaka Univ. (Japan)
RIKEN (Japan)
Japan Agency for Medical Research and Development (Japan)
Takumasa Sekiya, Osaka Univ. (Japan)
Den Ka, Osaka Univ. (Japan)
Hiroyuki Yamakoshi, RIKEN (Japan)
RIKEN (Japan)
Japan Agency for Medical Research and Development (Japan)
Takumasa Sekiya, Osaka Univ. (Japan)
Den Ka, Osaka Univ. (Japan)
Hiroyuki Yamakoshi, RIKEN (Japan)
Kosuke Dodo, RIKEN (Japan)
Japan Agency for Medical Research and Development (Japan)
Mikiko Sodeoka, RIKEN (Japan)
Japan Agency for Medical Research and Development (Japan)
Satoshi Kawata, Osaka Univ. (Japan)
Katsumasa Fujita, Osaka Univ. (Japan)
Japan Agency for Medical Research and Development (Japan)
Japan Agency for Medical Research and Development (Japan)
Mikiko Sodeoka, RIKEN (Japan)
Japan Agency for Medical Research and Development (Japan)
Satoshi Kawata, Osaka Univ. (Japan)
Katsumasa Fujita, Osaka Univ. (Japan)
Japan Agency for Medical Research and Development (Japan)
Published in SPIE Proceedings Vol. 10046:
Visualizing and Quantifying Drug Distribution in Tissue
Kin Foong Chan; Conor L. Evans, Editor(s)
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