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Proceedings Paper

Real-time monitoring the distribution of antibody-photo-absorber conjugates during photoimmunotherapy in vivo (Conference Presentation)
Author(s): Qinggong Tang; Jonathan Lin; Tadanobu Nagaya; Yi Liu; Hisataka Kobayashi; Yu Chen

Paper Abstract

Photo-immunotherapy (PIT) is an emerging low-side-effect cancer therapy based on monoclonal antibody (mAb) conjugated with a near-infrared (NIR) phthalocyanine dye IRDye700DX (IR700 is not only fluorescent which can be used as an imaging agent, but also phototoxic) that induces rapid cell death after exposure to NIR light. PIT induces highly-selective cancer cell death while leaving most of tumor blood vessels unharmed, leading to an effect termed super-enhanced permeability and retention (SUPR), which significantly improve the effectiveness of anti-cancer drug. Currently, the therapeutic effects of PIT were monitored using IR700 fluorescent signal based on macroscopic fluorescence reflectance imager, which lacks the resolution and depth information to reveal the intra-tumor heterogeneity of mAb-IR700 distribution. We developed a minimally-invasive two-channel fluorescence fiber imaging system by combining the traditional fluorescence imaging microscope with two imaging fiber bundles (~0.85 mm) to monitor mAb-IR700 distribution and therapeutic effects during PIT at different intra-tumor locations (e.g. tumor periphery vs. tumor rim) in situ and in real time simutaneously, thereby enabling evaluation of the therapeutic effects in vivo and optimization of treatment regimens accordingly. Experiments were carried out on ten mice. The average fluorescence intensity recovery after PIT in tumor rim is 91.50% while 100.63% in tumor periphery. Significantly higher fluorescence redistribution (P=0.0371) in tumor periphery than tumor rim after PIT treatment were observed. In order to verify the results, two-photon microscopy combining with micro-prism was also used to record the mAb-IR700 distribution at different depth locations of the tumor during PIT.

Paper Details

Date Published: 19 April 2017
PDF: 1 pages
Proc. SPIE 10047, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XXVI, 100470V (19 April 2017); doi: 10.1117/12.2253355
Show Author Affiliations
Qinggong Tang, Univ. of Maryland, College Park (United States)
Jonathan Lin, Univ. of Maryland, College Park (United States)
Tadanobu Nagaya, National Cancer Institute (United States)
Yi Liu, Univ. of Maryland, College Park (United States)
Hisataka Kobayashi, National Cancer Institute (United States)
National Institute of Health (United States)
Yu Chen, Univ. of Maryland, College Park (United States)

Published in SPIE Proceedings Vol. 10047:
Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XXVI
David H. Kessel; Tayyaba Hasan, Editor(s)

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