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Proceedings Paper

Cost-effective and label-free holographic biosensor for detection of herpes simplex virus (Conference Presentation)
Author(s): Aniruddha Ray; Ha Ho; Mustafa Daloglu; Avee Torres; Euan McLeod; Aydogan Ozcan

Paper Abstract

Herpes is one of the most widespread sexually transmitted viral diseases. Timely detection of Herpes Simplex Virus (HSV) can help prevent the rampant spreading of the virus. Current detection techniques such as viral culture, immuno-assays or Polymerase-Chain-Reaction, are time extensive and require expert handling. Here we present a field-portable, easy-to-use, and cost-effective biosensor for the detection of HSV based on holographic imaging. The virus is first captured from a target solution onto specifically developed substrates, prepared by coating glass coverslips with HSV-specific antibodies, and imaged using a lensfree holographic microscope. Several light-emitting-diodes (LEDs), coupled to multi-mode optical-fibers, are used to illuminate the sample containing the viruses. A micro-controller is used to activate the LEDs one at a time and in-line holograms are recorded using a CMOS imager placed immediately above the substrate. These sub-pixel shifted holograms are used to generate a super-resolved hologram, which is reconstructed to obtain the phase and amplitude images of the viruses. The signal of the viruses is enhanced using self-assembled PEG-based nanolenses, formed around the viral particles. Based on the phase information of the reconstructed images we can estimate the size of the viral particles, with an accuracy of ± 11 nm, as well as quantify the viral load. The limit-of-detection of this system is estimated to be <500 viral copies per 100 μL sample volume that is imaged over 30 mm^2 field-of-view. This holographic microscopy based biosensor is label-free, cost-effective and field-portable, providing results in 2 hours, including sample preparation and imaging time.

Paper Details

Date Published: 20 April 2017
PDF: 1 pages
Proc. SPIE 10055, Optics and Biophotonics in Low-Resource Settings III, 100550D (20 April 2017); doi: 10.1117/12.2251841
Show Author Affiliations
Aniruddha Ray, Univ. of California, Los Angeles (United States)
Ha Ho, Univ. of California, Los Angeles (United States)
Mustafa Daloglu, Univ. of California, Los Angeles (United States)
Avee Torres, Univ. of California, Los Angeles (United States)
Euan McLeod, The Univ. of Arizona (United States)
Aydogan Ozcan, Univ. of California, Los Angeles (United States)


Published in SPIE Proceedings Vol. 10055:
Optics and Biophotonics in Low-Resource Settings III
David Levitz; Aydogan Ozcan; David Erickson, Editor(s)

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