Share Email Print
cover

Proceedings Paper

Short infrared (IR) laser pulses can induce nanoporation
Format Member Price Non-Member Price
PDF $17.00 $21.00

Paper Abstract

Short infrared (IR) laser pulses on the order of hundreds of microseconds to single milliseconds with typical wavelengths of 1800-2100 nm, have shown the capability to reversibly stimulate action potentials (AP) in neuronal cells. While the IR stimulation technique has proven successful for several applications, the exact mechanism(s) underlying the AP generation has remained elusive. To better understand how IR pulses cause AP stimulation, we determined the threshold for the formation of nanopores in the plasma membrane. Using a surrogate calcium ion, thallium, which is roughly the same shape and charge, but lacks the biological functionality of calcium, we recorded the flow of thallium ions into an exposed cell in the presence of a battery of channel antagonists. The entry of thallium into the cell indicated that the ions entered via nanopores. The data presented here demonstrate a basic understanding of the fundamental effects of IR stimulation and speculates that nanopores, formed in response to the IR exposure, play an upstream role in the generation of AP.

Paper Details

Date Published: 9 March 2016
PDF: 7 pages
Proc. SPIE 9690, Clinical and Translational Neurophotonics; Neural Imaging and Sensing; and Optogenetics and Optical Manipulation, 96901L (9 March 2016); doi: 10.1117/12.2214892
Show Author Affiliations
Caleb C. Roth, The Univ. of Texas Health Science Ctr. at San Antonio (United States)
Engility Corp. (United States)
Ronald A. Barnes Jr., Oak Ridge Institute for Science and Education (United States)
Bennett L. Ibey, Air Force Research Lab. (United States)
Randolph D. Glickman, The Univ. of Texas Health Science Ctr. at San Antonio (United States)
Hope T. Beier, Air Force Research Lab. (United States)


Published in SPIE Proceedings Vol. 9690:
Clinical and Translational Neurophotonics; Neural Imaging and Sensing; and Optogenetics and Optical Manipulation
Steen J. Madsen; E. Duco Jansen; Samarendra K. Mohanty; Nitish V. Thakor; Qingming Luo; Victor X. D. Yang, Editor(s)

© SPIE. Terms of Use
Back to Top
PREMIUM CONTENT
Sign in to read the full article
Create a free SPIE account to get access to
premium articles and original research
Forgot your username?
close_icon_gray