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Proceedings Paper

High-spatial-resolution mapping of the oxygen concentration in cortical tissue (Conference Presentation)
Author(s): Rajeshwer S. Jaswal; Mohammad A. Yaseen; Buyin Fu; David A. Boas; Sava Sakadžic

Paper Abstract

Due to a lack of imaging tools for high-resolution imaging of cortical tissue oxygenation, the detailed maps of the oxygen partial pressure (PO2) around arterioles, venules, and capillaries remain largely unknown. Therefore, we have limited knowledge about the mechanisms that secure sufficient oxygen delivery in microvascular domains during brain activation, and provide some metabolic reserve capacity in diseases that affect either microvascular networks or the regulation of cerebral blood flow (CBF). To address this challenge, we applied a Two-Photon PO2 Microscopy to map PO2 at different depths in mice cortices. Measurements were performed through the cranial window in the anesthetized healthy mice as well as in the mouse models of microvascular dysfunctions. In addition, microvascular morphology was recorded by the two-photon microscopy at the end of each experiment and subsequently segmented. Co-registration of the PO2 measurements and exact microvascular morphology enabled quantification of the tissue PO2 dependence on distance from the arterioles, capillaries, and venules at various depths. Our measurements reveal significant spatial heterogeneity of the cortical tissue PO2 distribution that is dominated by the high oxygenation in periarteriolar spaces. In cases of impaired oxygen delivery due to microvascular dysfunction, significant reduction in tissue oxygenation away from the arterioles was observed. These tissue domains may be the initial sites of cortical injury that can further exacerbate the progression of the disease.

Paper Details

Date Published: 26 April 2016
PDF: 1 pages
Proc. SPIE 9690, Clinical and Translational Neurophotonics; Neural Imaging and Sensing; and Optogenetics and Optical Manipulation, 96901G (26 April 2016); doi: 10.1117/12.2212716
Show Author Affiliations
Rajeshwer S. Jaswal, Athinoula A. Martinos Ctr. for Biomedical Imaging (United States)
Mohammad A. Yaseen, Athinoula A. Martinos Ctr. for Biomedical Imaging (United States)
Buyin Fu, Massachusetts General Hospital (United States)
David A. Boas, Athinoula A. Martinos Ctr. for Biomedical Imaging (United States)
Sava Sakadžic, Athinoula A. Martinos Ctr. for Biomedical Imaging (United States)


Published in SPIE Proceedings Vol. 9690:
Clinical and Translational Neurophotonics; Neural Imaging and Sensing; and Optogenetics and Optical Manipulation
Steen J. Madsen; E. Duco Jansen; Samarendra K. Mohanty; Nitish V. Thakor; Qingming Luo; Victor X. D. Yang, Editor(s)

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