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Proceedings Paper

Raman spectroscopy and oral exfoliative cytology
Author(s): Aditi Sahu; Nupur Shah; Manoj Mahimkar; Mandavi Garud; Sandeep Pagare; Sudhir Nair; C. Murali Krishna
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Paper Abstract

Early detection of oral cancers can substantially improve disease-free survival rates. Ex vivo and in vivo Raman spectroscopic (RS) studies on oral cancer have demonstrated the applicability of RS in identifying not only malignant and premalignant conditions but also cancer-field-effects: the earliest events in oral carcinogenesis. RS has also been explored for cervical exfoliated cells analysis. Exfoliated cells are associated with several advantages like non-invasive sampling, higher patient compliance, transportation and analysis at a central facility: obviating need for on-site instrumentation. Thus, oral exfoliative cytology coupled with RS may serve as a useful adjunct for oral cancer screening. In this study, exfoliated cells from healthy controls with and without tobacco habits, premalignant lesions (leukoplakia and tobacco-pouch-keratosis) and their contralateral mucosa were collected using a Cytobrush. Cells were harvested by vortexing and centrifugation at 6000 rpm. The cellular yield was ascertained using Neubauer’s chamber. Cell pellets were placed on a CaF2 window and Raman spectra were acquired using a Raman microprobe (40X objective) coupled HE-785 Raman spectrometer. Approximately 7 spectra were recorded from each pellet, following which pellet was smeared onto a glass slide, fixed in 95% ethanol and subjected to Pap staining for cytological diagnosis (gold standard). Preliminary PC-LDA followed by leave-one-out cross validation indicate delineation of cells from healthy and all pathological conditions. A tendency of classification was also seen between cells from contralateral, healthy tobacco and site of premalignant lesions. These results will be validated by cytological findings, which will serve as the basis for building standard models of each condition.

Paper Details

Date Published: 4 March 2014
PDF: 4 pages
Proc. SPIE 8926, Photonic Therapeutics and Diagnostics X, 89262N (4 March 2014); doi: 10.1117/12.2052794
Show Author Affiliations
Aditi Sahu, Advanced Ctr. for Treatment, Research and Education in Cancer (India)
Nupur Shah, Advanced Ctr. for Treatment, Research and Education in Cancer (India)
Manoj Mahimkar, Advanced Ctr. for Treatment, Research and Education in Cancer (India)
Mandavi Garud, D. Y. Patil Dental College (India)
Sandeep Pagare, D. Y. Patil Dental College (India)
Sudhir Nair, Advanced Ctr. for Treatment, Research and Education in Cancer (India)
C. Murali Krishna, Advanced Ctr. for Treatment, Research and Education in Cancer (India)

Published in SPIE Proceedings Vol. 8926:
Photonic Therapeutics and Diagnostics X
Bernard Choi; Hyun Wook Kang; Brian J. F. Wong M.D.; Guillermo J. Tearney M.D.; Andreas Mandelis; Nikiforos Kollias; Kenton W. Gregory M.D.; Justus F. Ilgner M.D.; Haishan Zeng; Laura Marcu, Editor(s)

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