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Proceedings Paper

Simulation-based investigation of the three-dimensional distribution of fluorescence and photobleaching in multi-photon excited samples
Author(s): Imre B. Juhász; Árpád I. Csurgay
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Paper Abstract

We present a numerical study on the spatial distribution of fluorescence and photobleaching occurring in samples subject to multi-photon excitation. We developed a simulation model and implemented a simulator program. Its quantitative predictions can help to find the optimal operating parameters (such as laser power, pulse length, pulse repetition rate) of the two-photon microscope to reach higher image quality, to reduce undesired photobleaching, and to pave the way for optimized photoswitching-based super-resolution imaging. Conversely, the simulator might also be useful when photodynamic parameters are searched for. Furthermore, such simulations can promote the evaluation of the results of other fluorescence-based techniques [e.g. fluorescence recovery after photobleaching (FRAP) measurements]. The photodynamic model of the fluorophore contains a ground state, an excited state, a triplet state, and several photobleached states; the state transitions are characterized by absorption cross sections and lifetimes. The sample is modeled as a fluorophore solution divided into cubic cells among which diffusion takes place. The illumination is simulated as a focused laser pulse train described by a pulsed Gaussian beam. As a demonstration of the capabilities of the simulator, an example is presented that reveals the spatial distribution of photon emission in the sample investigated by a two-photon microscope in the case of different laser and photobleaching parameters, assuming one-photon absorption induced photobleaching. The simulation demonstrates quantitatively how photobleaching affects the spatial distribution of fluorescence and the resolution of the microscope.

Paper Details

Date Published: 1 May 2014
PDF: 10 pages
Proc. SPIE 9131, Optical Modelling and Design III, 91310S (1 May 2014); doi: 10.1117/12.2052243
Show Author Affiliations
Imre B. Juhász, Pázmány Péter Catholic Univ. (Hungary)
Árpád I. Csurgay, Pázmány Péter Catholic Univ. (Hungary)

Published in SPIE Proceedings Vol. 9131:
Optical Modelling and Design III
Frank Wyrowski; John T. Sheridan; Jani Tervo; Youri Meuret, Editor(s)

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