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Proceedings Paper

Effect of LLLT on the level of ATP and ROS from organ of corti cells
Author(s): ChungKu Rhee M.D.; So-Young Chang; Jin-Chul Ahn; Myung-Whan Suh; Jae Yun Jung
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Paper Abstract

It is well established that ototoxic antibiotics and acoustic trauma can damage cochlear hair cells and cause hearing loss. Previous studies using transcanal LLLT (Low level laser therapy) showed that LLLT can promote recovery of hearing thresholds and cochlear hair cells. However, its mechanism has not been studied. Aim: The aim of this study is to investigate the mechanism of hearing recovery from gentamicin induced ototoxic hearing loss by LLLT. Methods: HEI- OC1 (House ear institute organ of Corti) cells were cultured for 18 hours and ototoxicity was induced by gentamicin (GM) treatment to the cells. Cultured cells were divided into 6 groups, No treatment control, LLLT only, GM 6.6 mM and GM 13.1 mM, GM 6.6 mM+LLLT and GM 13.1 mM+LLLT cells. LD laser 808 nm, 15 mW, was irradiated to the cultured cells for 15 min, at 4 hours after GM treatment to the cells. ATP was assayed using the ATP assay Kit. ROS was measured using confocal microscope after application of H2DCFDA dye. Results: ATP was decreased in GM 13.1 mM cells and increased in LLLT only cells and GM 13.1 mM+LLLT cells compared to control and 13.1 mM cells. ROS was increased in GM 6.6 mM and GM 13.1 mM cells, and decreased in GM 6.6 mM+LLLT and GM 13.1 mM+LLLT cells compared to GM 6.6 and 13.1 mM cells immediately after laser irradiation. Conclusion: This study demonstrated that LLLT on GM treated HEI-OC1 cells increased ATP and decreased ROS that may contribute to the recovery of hearing.

Paper Details

Date Published: 4 March 2014
PDF: 7 pages
Proc. SPIE 8926, Photonic Therapeutics and Diagnostics X, 89261Y (4 March 2014); doi: 10.1117/12.2041438
Show Author Affiliations
ChungKu Rhee M.D., Dankook Univ. Hospital (Korea, Republic of)
So-Young Chang, Dankook Univ. Hospital (Korea, Republic of)
Jin-Chul Ahn, Dankook Univ. Hospital (Korea, Republic of)
Myung-Whan Suh, Seoul National Univ. (Korea, Republic of)
Jae Yun Jung, Dankook Univ. (Korea, Republic of)

Published in SPIE Proceedings Vol. 8926:
Photonic Therapeutics and Diagnostics X
Bernard Choi; Hyun Wook Kang; Brian J. F. Wong M.D.; Guillermo J. Tearney M.D.; Andreas Mandelis; Nikiforos Kollias; Kenton W. Gregory M.D.; Justus F. Ilgner M.D.; Haishan Zeng; Laura Marcu, Editor(s)

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