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Proceedings Paper

Tumor site prediction using spatiotemporal detection of subclinical hyperemia in experimental photocarcinogenesis
Author(s): Raymond L. Konger; Zhengbin Xu; Ravi P. Sahu; Young L. Kim
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Paper Abstract

We demonstrate that a spatial and temporal analysis of subclinical hyperemia reliably predicts specific areas at high risk for skin tumor development during photocarcinogenesis. To determine detailed spatiotemporal patterns of inflammatory angiogenesis foci in a relatively large area, we developed a mesoscopic (between microscopic and macroscopic) imaging approach. This method relies on our earlier finding that the combination of a spectral analysis of hemoglobin with diffuse-light-suppressed imaging can increase the image resolution, contrast and penetration depth to visualize microvasculature Hgb content in the large tissue area. In our recent study, SKH1 hairless albino mice were irradiated for 10 weeks with a carcinogen dose of UVB. Using our newly developed mesoscopic imaging methods, we imaged the mice over 20 - 30 weeks after stopping UVB, and excised hyperemic/non-hyperemic areas at several different timepoints. We show that persistent hyperemic foci can predict future tumor formation. In particular, our imaging approach allows us to assess the spatial and temporal extent of subclinical inflammatory foci, which in turn can predict sites of future overlying tumor formation. In addition, although COX-2 inhibitors are known to suppress skin cancer development in humans, it remains unclear whether the chemopreventive activity of COX-2 inhibitors are chiefly attributable to their anti-inflammatory effects. Our study provides evidence that subclinical subepithelial inflammatory foci occur prior to overt tumor formation, and that these areas are highly predictive for future tumor formation, that celecoxib’s ability to suppress tumorigenesis is tightly linked to its ability to reduce the area of subclinical inflammatory foci.

Paper Details

Date Published: 4 March 2014
PDF: 8 pages
Proc. SPIE 8926, Photonic Therapeutics and Diagnostics X, 89260Z (4 March 2014);
Show Author Affiliations
Raymond L. Konger, Indiana Univ. School of Medicine (United States)
Zhengbin Xu, Purdue Univ. (United States)
Ravi P. Sahu, Indiana Univ. School of Medicine (United States)
Young L. Kim, Purdue Univ. (United States)

Published in SPIE Proceedings Vol. 8926:
Photonic Therapeutics and Diagnostics X
Bernard Choi; Hyun Wook Kang; Brian J. F. Wong M.D.; Guillermo J. Tearney M.D.; Andreas Mandelis; Nikiforos Kollias; Kenton W. Gregory M.D.; Justus F. Ilgner M.D.; Haishan Zeng; Laura Marcu, Editor(s)

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