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Proceedings Paper

Micro-Raman spectroscopy studies of changes in lipid composition in breast and prostate cancer cells treated with MPA and R1881 hormones
Author(s): Mariana C. Potcoava; Gregory L. Futia; Jessica Aughenbaugh; Isabel Schlaepfer; Emily A. Gibson
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Paper Abstract

Increasing interest in the role of lipids in cancer cell proliferation or resistance to drug therapies has motivated the need to develop better tools for cellular lipid analysis. Quantification of lipids in cells is typically done by destructive chromatography protocols that do not provide spatial information on lipid distribution and prevent dynamic live cell studies. Methods that allow the analysis of lipid content in live cells is therefore of great importance for research. Using Raman micro-spectroscopy we investigated whether the female hormone medroxyprogesterone acetate (MPA) and the synthetic androgen R1881 affect the lipid expression in breast (T47D) and prostate (LNCaP) cancer cells. Differences were noted in the spectral regions at 830-1800 cm-1 and 2800-3000 cm-1 when comparing different drug treatments. Significant changes were noticed for saturated (1063 - 1125 cm-1, 1295 cm-1 and 1439 cm-1), unsaturated (1262 cm-1 and 1656 cm-1, and 1720 - 1748 cm-1) chemical bonds, suggesting that the composition of the lipid droplets was changed by the hormone treatments. Also, significant differences were observed in the high frequency regions of lipids and proteins at 2851 cm-1 and around 2890 cm-1. Principal component analysis with Linear Discriminant Analysis (PCA-LDA) of the Raman spectra was able to differentiate between cancer cells that were treated with MPA, R1881 or vehicle (P < 0.05). Future work includes analysis to determine exact lipid composition and concentrations as well as development of clinical techniques to characterize differences in patient tumor lipid profiles to determine response to drug treatment and prognosis.

Paper Details

Date Published: 4 March 2014
PDF: 7 pages
Proc. SPIE 8939, Biomedical Vibrational Spectroscopy VI: Advances in Research and Industry, 89390I (4 March 2014);
Show Author Affiliations
Mariana C. Potcoava, Univ. of Colorado, Denver (United States)
Gregory L. Futia, Univ. of Colorado, Denver (United States)
Jessica Aughenbaugh, Univ. of Colorado, Denver (United States)
Isabel Schlaepfer, Univ. of Colorado, Denver (United States)
Emily A. Gibson, Univ. of Colorado, Denver (United States)

Published in SPIE Proceedings Vol. 8939:
Biomedical Vibrational Spectroscopy VI: Advances in Research and Industry
Anita Mahadevan-Jansen; Wolfgang Petrich, Editor(s)

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