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Proceedings Paper

Photodynamic therapy induces epidermal thickening in hairless mice skin: an optical coherence tomography assessment
Author(s): Ana Elisa S. Jorge; Carolina P. Campos; Anderson Z. Freitas; Vanderlei S. Bagnato
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Paper Abstract

Photodynamic therapy (PDT) promotes skin improvement according to many practitioners, however the immediately in vivo assessment of its response remains clinically inaccessible. As a non-invasive modality, optical coherence tomography (OCT) has been shown a feasible optical diagnostic technique that provides images in real time, avoiding tissue biopsies. For this reason, our investigation focused on evaluates the PDT effect on a rodent model by means of OCT. Therefore, a normal hairless mouse skin has undergone a single-session PDT, which was performed with topical 5- aminolevulinic acid (ALA) cream using a red (630 nm) light emitting diode (LED) which reached the light dose of 75 J/cm2. As the optical imaging tool, an OCT (930 nm) with axial resolution of 6.0 microns in air was used, generating images with contact to the mouse skin before, immediately after, 24 hours, and 2 weeks after the correspondent procedure. Our result demonstrates that, within 24 hours after ALA-PDT, the mouse skin from the PDT group has shown epidermal thickness (ET), which has substantially increased after 2 weeks from the treatment day. Moreover, the skin surface has become evener after ALA-PDT. Concluding, this investigation demonstrates that the OCT is a feasible and reliable technique that allows real-time cross-sectional imaging of skin, which can quantify an outcome and predict whether the PDT reaches its goal.

Paper Details

Date Published: 4 March 2014
PDF: 6 pages
Proc. SPIE 8934, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XVIII, 89343N (4 March 2014); doi: 10.1117/12.2040443
Show Author Affiliations
Ana Elisa S. Jorge, Univ. de São Paulo (Brazil)
Carolina P. Campos, Univ. de São Paulo (Brazil)
Anderson Z. Freitas, Instituto de Pesquisas Energéticas e Nucleares (Brazil)
Vanderlei S. Bagnato, Univ. de São Paulo (Brazil)

Published in SPIE Proceedings Vol. 8934:
Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XVIII
Joseph A. Izatt; James G. Fujimoto; Valery V. Tuchin, Editor(s)

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