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Proceedings Paper

Fourier transform infrared spectroscopic imaging identifies early biochemical markers of tissue damage
Author(s): Vishal K. Varma; Samuel Ohlander; Peter Nguyen; Christopher Vendryes; Sujeeth Parthiban; Blake Hamilton; M. Chad Wallis; Andre Kajdacsy-Balla; Blake Hannaford; Thomas Lendvay; James M. Hotaling; Michael J. Walsh
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Paper Abstract

Fourier Transform Infrared (FT-IR) spectroscopic imaging can allow for the rapid imaging of tissue biochemistry in a label-free and non-perturbing fashion. With the rapid adoption of new minimally invasive surgery (MIS) technologies over the last 20 years, adequate skill to safely and effectively use these technologies may not be achieved and risk of undue physical pressure being placed on tissues is a concern. Previous work has demonstrated that a number of histological stains can detect tissue damage, however, this process requires the initiation and progression of a signaling cascade that results in the epitope of interest being expressed. We proposed to identify the early biochemical markers associated with physical tissue damage from applied forces, thus not requiring transcriptional and translational protein synthesis as traditional immunohistochemistry does. To demonstrate that FT-IR can measure biochemical changes in tissues that have undergone physical force, we took ex-vivo lamb’s liver that had been freshly excised and applied varying levels of physical pressure (0kPa to 30kPa). Tissues were then formalin-fixed, paraffin-embedded, and sectioned on to glass for H and E staining to identify damage and on to an IR slide for FT-IR imaging. Regions of interest containing hepatocytes were identified and average FT-IR spectra were extracted from the damaged and undamaged livers. FT-IR spectra showed clear biochemical changes associated with tissue damage. In addition, chemical changes could be observed proceeding histological changes observed when using conventional staining approaches.

Paper Details

Date Published: 4 March 2014
PDF: 7 pages
Proc. SPIE 8939, Biomedical Vibrational Spectroscopy VI: Advances in Research and Industry, 89390T (4 March 2014); doi: 10.1117/12.2040282
Show Author Affiliations
Vishal K. Varma, Univ. of Illinois at Chicago (United States)
Samuel Ohlander, Univ. of Illinois at Chicago (United States)
Peter Nguyen, Univ. of Illinois at Chicago (United States)
Christopher Vendryes, Univ. of Illinois at Chicago (United States)
Sujeeth Parthiban, Univ. of Illinois at Chicago (United States)
Blake Hamilton, Univ. of Utah (United States)
M. Chad Wallis, Univ. of Utah (United States)
Andre Kajdacsy-Balla, Univ. of Illinois at Chicago (United States)
Blake Hannaford, Univ. of Washington (United States)
Thomas Lendvay, Univ. of Washington (United States)
James M. Hotaling, Univ. of Utah (United States)
Michael J. Walsh, Univ. of Illinois at Chicago (United States)

Published in SPIE Proceedings Vol. 8939:
Biomedical Vibrational Spectroscopy VI: Advances in Research and Industry
Anita Mahadevan-Jansen; Wolfgang Petrich, Editor(s)

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