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Proceedings Paper

Quantitative fiber-optic Raman spectroscopy for tissue Raman measurements
Author(s): Shiyamala Duraipandian; Mads Bergholt; Wei Zheng; Zhiwei Huang
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Paper Abstract

Molecular profiling of tissue using near-infrared (NIR) Raman spectroscopy has shown great promise for in vivo detection and prognostication of cancer. The Raman spectra measured from the tissue generally contain fundamental information about the absolute biomolecular concentrations in tissue and its changes associated with disease transformation. However, producing analogues tissue Raman spectra present a great technical challenge. In this preliminary study, we propose a method to ensure the reproducible tissue Raman measurements and validated with the in vivo Raman spectra (n=150) of inner lip acquired using different laser powers (i.e., 30 and 60 mW). A rapid Raman spectroscopy system coupled with a ball-lens fiber-optic Raman probe was utilized for tissue Raman measurements. The investigational results showed that the variations between the spectra measured with different laser powers are almost negligible, facilitating the quantitative analysis of tissue Raman measurements in vivo.

Paper Details

Date Published: 4 March 2014
PDF: 6 pages
Proc. SPIE 8939, Biomedical Vibrational Spectroscopy VI: Advances in Research and Industry, 89390V (4 March 2014); doi: 10.1117/12.2039576
Show Author Affiliations
Shiyamala Duraipandian, National Univ. of Singapore (Singapore)
Mads Bergholt, National Univ. of Singapore (Singapore)
Wei Zheng, National Univ. of Singapore (Singapore)
Zhiwei Huang, National Univ. of Singapore (Singapore)

Published in SPIE Proceedings Vol. 8939:
Biomedical Vibrational Spectroscopy VI: Advances in Research and Industry
Anita Mahadevan-Jansen; Wolfgang Petrich, Editor(s)

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