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Proceedings Paper

Prognostic prospective of laser induced fluorescence as an objective tool to evaluate collagen deposition in thermal wounds: an ex vivo study
Author(s): Vijendra Prabhu; Anusha Acharya; Satish Bola Sadashiva Rao; Pramod Kumar; Anurag Sharan; Krishna Kishore Mahato
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Paper Abstract

We aimed to investigate the predicative potential of laser induced fluorescence (LIF) in assessing collagen synthesis during wound healing progression following thermal injury. Granulation tissues of thermal wounds before grafting were collected and LIF measurements were carried out by exciting them with 325 nm to measure collagen. Biochemical estimation of the hydroxyproline was also carried out. Spectroscopic assessment & biochemical estimation of the wound granulation tissues displayed varied collagen levels. It can be concluded that LIF has the potential to be used as an objective tool to qualitatively determine the collagen levels in wound granulation tissues.

Paper Details

Date Published: 4 March 2014
PDF: 7 pages
Proc. SPIE 8926, Photonic Therapeutics and Diagnostics X, 892608 (4 March 2014); doi: 10.1117/12.2039416
Show Author Affiliations
Vijendra Prabhu, Manipal Univ. (India)
Anusha Acharya, Manipal Univ. (India)
Satish Bola Sadashiva Rao, Manipal Univ. (India)
Pramod Kumar, Manipal Univ. (India)
Anurag Sharan, Manipal Univ. (India)
Krishna Kishore Mahato, Manipal Univ. (India)

Published in SPIE Proceedings Vol. 8926:
Photonic Therapeutics and Diagnostics X
Bernard Choi; Hyun Wook Kang; Brian J. F. Wong M.D.; Guillermo J. Tearney M.D.; Andreas Mandelis; Nikiforos Kollias; Kenton W. Gregory M.D.; Justus F. Ilgner M.D.; Haishan Zeng; Laura Marcu, Editor(s)

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