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Proceedings Paper

Analysis of virus infected cell by Raman spectroscopy and transmission electron microscopy
Author(s): Kamila Moor; Kiyoshi Ohtani; Diyas Myrzakozha; Orik Zhanserkenova; Bibin B. Andriana; Hidetoshi Sato
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Paper Abstract

Raman spectroscopy is a promising tool for detection of virus infection in live cells. In the present study, we demonstrate its feasibility to observe dynamic reaction of the live cell infected by virus. The Raman spectra of the adenovirus infected live cell (293 HEK) are analyzed by comparing with those of control cells. Principal component analysis (PCA) is employed also to analyze the spectra in detail. A band at 1650 cm-1 increases its intensity in the spectra measured at 24 hours after the virus infection. The infection of the virus is also examined by immune-staining and transmission electron microscope (TEM), and the virus infection is confirmed with these method also. It should be noted that the present technique does not require specifying the type of virus in advance.

Paper Details

Date Published: 4 March 2014
PDF: 7 pages
Proc. SPIE 8939, Biomedical Vibrational Spectroscopy VI: Advances in Research and Industry, 893902 (4 March 2014); doi: 10.1117/12.2038020
Show Author Affiliations
Kamila Moor, Kwansei Gakuin Univ. (Japan)
Kiyoshi Ohtani, Kwansei Gakuin Univ. (Japan)
Diyas Myrzakozha, Kazakstan National Agrarian Univ. (Kazakhstan)
Orik Zhanserkenova, Kazakstan National Agrarian Univ. (Kazakhstan)
Bibin B. Andriana, Kwansei Gakuin Univ. (Japan)
Hidetoshi Sato, Kwansei Gakuin Univ. (Japan)

Published in SPIE Proceedings Vol. 8939:
Biomedical Vibrational Spectroscopy VI: Advances in Research and Industry
Anita Mahadevan-Jansen; Wolfgang Petrich, Editor(s)

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