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Proceedings Paper

Quantitative fluorescence molecular imaging in highly light-absorbing melanomas using a dual-tracer kinetic modeling normalization method
Author(s): Kenneth M. Tichauer; Stephen C. Kanick; Sophie J. Deharvengt; Kimberley S. Samkoe; Tayyaba Hasan; Radu V. Stan; Brian W. Pogue
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Paper Abstract

Tissues with high light absorption, such as melanomas, present a significant challenge to fluorescence imaging approaches that seek to estimate molecular expression in vivo, since any fluorescence originating in the tissue will suffer substantial attenuation prior to detection. This can lead to sizable underestimations in estimated fluorescent tracer concentration in these tissues using conventional fluorescence imaging. In this study, a dual-tracer fluorescence imaging approach was employed to correct for severe tissue absorption by 1) using simultaneous injection and imaging of an untargeted tracer to normalize tissue absorption effects on the targeted tracer, and 2) using kinetic modeling that capitalizes on subtle differences in the dynamics of targeted and untargeted tracer uptake to quantify targeted molecule concentrations in the high absorbing tissue. Monte Carlo simulation and kinetic models demonstrated that the effect of optical properties on the approach could be eliminated by a pixel-by-pixel normalization of the targeted and untargeted tracer uptakes prior to 5 min post-tracer injection for fluorescence planar dynamic imaging.

Paper Details

Date Published: 4 March 2014
PDF: 7 pages
Proc. SPIE 8926, Photonic Therapeutics and Diagnostics X, 89260T (4 March 2014); doi: 10.1117/12.2037424
Show Author Affiliations
Kenneth M. Tichauer, Illinois Institute of Technology (United States)
Stephen C. Kanick, Thayer School of Engineering at Dartmouth (United States)
Sophie J. Deharvengt, Geisel School of Medicine (United States)
Kimberley S. Samkoe, Thayer School of Engineering at Dartmouth (United States)
Geisel School of Medicine (United States)
Tayyaba Hasan, Wellman Ctr. for Photomedicine, Harvard Medical School (United States)
Radu V. Stan, Geisel School of Medicine (United States)
Brian W. Pogue, Thayer School of Engineering at Dartmouth (United States)
Geisel School of Medicine (United States)
The Wellman Institute of Photomedicine, Massachusetts General Hospital (United States)

Published in SPIE Proceedings Vol. 8926:
Photonic Therapeutics and Diagnostics X
Bernard Choi; Hyun Wook Kang; Brian J. F. Wong M.D.; Guillermo J. Tearney M.D.; Andreas Mandelis; Nikiforos Kollias; Kenton W. Gregory M.D.; Justus F. Ilgner M.D.; Haishan Zeng; Laura Marcu, Editor(s)

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