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Proceedings Paper

Calcium and voltage imaging in arrhythmia models by high-speed microscopy
Author(s): C. de Mauro; C. A. Cecchetti; D. Alfieri; G. Borile; A. Urbani; M. Mongillo; F. S. Pavone
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Paper Abstract

Alterations in intracellular cardiomyocyte calcium handling have a key role in initiating and sustaining arrhythmias. Arrhythmogenic calcium leak from sarcoplasmic reticulum (SR) can be attributed to all means by which calcium exits the SR store in an abnormal fashion. Abnormal SR calcium exit maymanifest as intracellular Ca2+ sparks and/or Ca2+ waves. Ca2+ signaling in arrhythmogenesis has been mainly studied in isolated cardiomyocytes and given that the extracellular matrix influences both Ca2+ and membrane potential dynamics in the intact heart and underlies environmentally mediated changes, understanding how Ca2+ and voltage are regulated in the intact heart will represent a tremendous advancement in the understanding of arrhythmogenic mechanisms. Using novel high-speed multiphoton microscopy techinques, such as multispot and random access, we investigated animal models with inherited and acquired arrhythmias to assess the role of Ca2+ and voltage signals as arrhythmia triggers in cell and subcellular components of the intact heart and correlate these with electrophysiology.

Paper Details

Date Published: 4 March 2014
PDF: 6 pages
Proc. SPIE 8926, Photonic Therapeutics and Diagnostics X, 89262W (4 March 2014); doi: 10.1117/12.2036973
Show Author Affiliations
C. de Mauro, Light4Tech Firenze S.r.l. (Italy)
C. A. Cecchetti, Light4Tech Firenze S.r.l. (Italy)
D. Alfieri, Light4Tech Firenze S.r.l. (Italy)
G. Borile, Venetian Institute of Molecular Medicine (Italy)
A. Urbani, Venetian Institute of Molecular Medicine (Italy)
M. Mongillo, Venetian Institute of Molecular Medicine (Italy)
F. S. Pavone, Univ. degli Studi di Firenze (Italy)

Published in SPIE Proceedings Vol. 8926:
Photonic Therapeutics and Diagnostics X
Bernard Choi; Hyun Wook Kang; Brian J. F. Wong M.D.; Guillermo J. Tearney M.D.; Andreas Mandelis; Nikiforos Kollias; Kenton W. Gregory M.D.; Justus F. Ilgner M.D.; Haishan Zeng; Laura Marcu, Editor(s)

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