Share Email Print

Proceedings Paper

Optical biopsy on head and neck tissue using full-field OCT: a pilot study
Author(s): Frédéric De Leeuw; Anne Latrive; Odile Casiraghi; Malek Ferchiou; Fabrice Harms; Claude Boccara; Corinne Laplace-Builhé
Format Member Price Non-Member Price
PDF $17.00 $21.00

Paper Abstract

Here we evaluate the clinical value of Full-Field OCT imaging in the management of patients with Head and Neck cancers by making a reliable histological diagnosis on FFOCT images produced during preoperative procedure. FFOCT performs a true "virtual extemporaneous exam" that we want to compare to the gold standard (extemporaneous and conventional histology with H and E staining). This new optical technology could be useful when diagnosing a lesion, cancerous or precancerous, or at the time of its surgical management. Full-Field Optical Coherence Tomography virtually slices the tissue using white light interferometry to produce in-depth 2D images with an isotropic resolution around 1 micrometer. With such a high resolution FFOCT systems produce ”optical biopsy” images that are similar to that obtained with classical histology procedures, but without any staining and in only a few minutes. We imaged freshly excised samples from patients, of mouth, tongue, epiglottis and larynx tissues, both healthy and cancerous. FFOCT images were acquired and later compared with histology of the same samples. Common features were identified and characteristics of each tissue type were matched in order to form an image atlas for pathologist training. We were able to identify indicators of tumors such as heterogeneities in cell distribution, surrounding stroma, anomalous keratinization… In conclusion, FFOCT is a fast, non-invasive, non-destructive imaging tool that can be inserted into the pathology lab workflow and can provide a quick assessment of microscopic tissue architecture and content. Furthermore we are developing a similar system with a rigid endoscopic probe in order to do in vivo and in situ high-resolution imaging. Our probe could thus guide the surgeon in real time before and during excision and ensure a more precise gesture.

Paper Details

Date Published: 4 March 2014
PDF: 9 pages
Proc. SPIE 8926, Photonic Therapeutics and Diagnostics X, 892626 (4 March 2014); doi: 10.1117/12.2036959
Show Author Affiliations
Frédéric De Leeuw, Institut Gustave Roussy (France)
Univ. Paris-Sud, CNRS (France)
Anne Latrive, LLTECH SAS (France)
Odile Casiraghi, Institut Gustave Roussy (France)
Malek Ferchiou, Institut Gustave Roussy (France)
Fabrice Harms, LLTECH SAS (France)
Claude Boccara, Institut Langevin, ESPCI, Paris Tech (France)
Corinne Laplace-Builhé, Institut Gustave Roussy (France)
Univ. Paris-Sud, CNRS (France)

Published in SPIE Proceedings Vol. 8926:
Photonic Therapeutics and Diagnostics X
Bernard Choi; Hyun Wook Kang; Brian J. F. Wong M.D.; Guillermo J. Tearney M.D.; Andreas Mandelis; Nikiforos Kollias; Kenton W. Gregory M.D.; Justus F. Ilgner M.D.; Haishan Zeng; Laura Marcu, Editor(s)

© SPIE. Terms of Use
Back to Top
Sign in to read the full article
Create a free SPIE account to get access to
premium articles and original research
Forgot your username?