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Proceedings Paper

Peptide-mediated cellular delivery of semiconductor quantum dots
Author(s): Kelly Boeneman Gemmill; Markus Muttenthaler; James Delehanty; Jeff Deschamps; Kimihiro Susumu; Michael Stewart; Philip Dawson; Alan Huston; Igor Medintz
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Paper Abstract

CdSe/ZnS semiconductor quantum dots (QDs) are ideal materials for biological sensing and cellular imaging applications due to their superior photophysical properties in comparison to fluorescent proteins or dyes and their ease of conjugation to biological materials. We have previously developed a number of in vitro FRET based biosensors in the laboratory for detection of proteases and biological and chemical agents. We would like to expand these biosensing capabilities into cellular systems, requiring development of QD cellular delivery techniques. Peptide mediated cellular delivery of QDs is ideal as peptides are small, easily conjugated to QDs, easily manipulated and synthesized, and can be designed with “handles” for further chemical conjugation with other cargo. Here we discuss four cell delivery peptides that facilitate QD uptake in live cells. Understanding these peptides will help us design better nanoparticle cellular delivery systems and advance our capabilities for in vivo biosensing.

Paper Details

Date Published: 29 May 2013
PDF: 6 pages
Proc. SPIE 8723, Sensing Technologies for Global Health, Military Medicine, and Environmental Monitoring III, 872311 (29 May 2013); doi: 10.1117/12.2015446
Show Author Affiliations
Kelly Boeneman Gemmill, U.S. Naval Research Lab. (United States)
Markus Muttenthaler, The Scripps Research Institute (United States)
James Delehanty, U.S. Naval Research Lab. (United States)
Jeff Deschamps, U.S. Naval Research Lab. (United States)
Kimihiro Susumu, U.S. Naval Research Lab. (United States)
Michael Stewart, U.S. Naval Research Lab. (United States)
Philip Dawson, The Scripps Research Institute (United States)
Alan Huston, U.S. Naval Research Lab. (United States)
Igor Medintz, U.S. Naval Research Lab. (United States)


Published in SPIE Proceedings Vol. 8723:
Sensing Technologies for Global Health, Military Medicine, and Environmental Monitoring III
Šárka O. Southern, Editor(s)

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