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Proceedings Paper

The cell-cycle dependence of the spectra of proliferating normal and neoplastic single cells using confocal resonance Raman microspectroscopy
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Paper Abstract

Confocal resonance Raman (RR) spectra were collected from single proliferating cells and analyzed to detect spectral patterns that are cell-cycle dependent, as a consequence of cellular proliferation — normal or abnormal. The cells’ biochemical age at each time point was confirmed by immunohistochemical staining to identify the presence or absence of cellular components that appear and/or disappear as the cells proceed through the cell-cycle. The RR spectra were collected and compared for each time point as the cells proceeded through the cell cycle to determine what spectral vibrational patterns are cell-cycle dependent. In this study, the question is whether the cell-cycle dependent RR spectral patterns of the vibrational modes observed in proliferating normal and neoplastic single cells are due to a state of cancer or are simply the consequences of the cells’ changing internal biochemistry due to the process of cellular proliferation --- normal or abnormal.

Paper Details

Date Published: 19 March 2013
PDF: 8 pages
Proc. SPIE 8577, Optical Biopsy XI, 85770C (19 March 2013); doi: 10.1117/12.2008738
Show Author Affiliations
Susie Boydston-White, Borough of Manhattan Community College (United States)
Cheng-Hui Liu, The City College of New York (United States)
Robert R. Alfano, The City College of New York (United States)


Published in SPIE Proceedings Vol. 8577:
Optical Biopsy XI
Robert R. Alfano; Stavros G. Demos, Editor(s)

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