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Proceedings Paper

Could near-infrared Raman spectroscopy be correlated with the METAVIR scores in liver lesions induced by hepatitis C virus?
Author(s): Marcio Cesar Reino Gaggini; Ricardo Scarparo Navarro; Aline Reis Stefanini; Rubens Sato Sano; Landulfo Silveira Jr.
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Paper Abstract

The liver is responsible for several basic functions in human body how the syntheses of the most main proteins and degradation process of toxins, drugs and alcohols. In present days, the viral hepatitis C is one of the highest causes of chronic hepatic illness worldwide, affecting around 3% of the world population. The liver biopsy is considered the gold standard for diagnosing hepatic fibrosis; however, the biopsies may be questioned because of potential sampling error, morbidity, possible mortality and relatively high costs. Spectroscopy techniques such as Raman spectroscopy have been used for diagnosis of human tissues, with favorable results. Raman spectroscopy has been employed to distinguish normal from hepatic lesions through spectral features mainly of proteins, nucleic acids and lipids. In this study, eleven patients with diagnoses of chronic hepatitis C underwent hepatic biopsies having two hepatic fragments collected: one was scored through METAVIR system and the other one was submitted to near-infrared Raman spectroscopy using a dispersive spectrometer (830 nm wavelength, 300 mW laser power and 20 s exposure time). Five spectra were collected in each fragment and submitted to Principal Components Analysis (PCA). Results showed a good correlation between the Raman spectroscopy features and the stage of hepatic fibrosis and inflammation. PCA showed that samples with higher degree of fibrosis presented higher amount of protein features (collagen), whereas samples of higher degree of inflammation presented higher features of hemoglobin, in accordance to the expected evolution of the chronic hepatitis. It has been found an important biomarker for the beginning of hepatic lesion (quinone) with a spectral feature at 1595 cm-1.

Paper Details

Date Published: 19 March 2013
PDF: 9 pages
Proc. SPIE 8577, Optical Biopsy XI, 85770F (19 March 2013);
Show Author Affiliations
Marcio Cesar Reino Gaggini, Univ. Camilo Castelo Branco (Brazil)
Irmandade da Santa Casa de Misericórdia de Fernandópolis (Brazil)
Ricardo Scarparo Navarro, Univ. Camilo Castelo Branco (Brazil)
Aline Reis Stefanini, Univ. Camilo Castelo Branco (Brazil)
Rubens Sato Sano, Irmandade da Santa Casa de Misericórdia de Fernandópolis (Brazil)
Landulfo Silveira Jr., Univ. Camilo Castelo Branco (Brazil)

Published in SPIE Proceedings Vol. 8577:
Optical Biopsy XI
Robert R. Alfano; Stavros G. Demos, Editor(s)

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