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Proceedings Paper

Effects of combined photochemical internalization and hyperthermia are sensitively dependent on radiant exposure
Author(s): Steen J. Madsen; Christina Schlazer; Aaron Andersen; Stephanie Molina; Henry Hirschberg
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Paper Abstract

Combination therapies of photochemical internalization (PCI) and hyperthermia (HT) were investigated in an in vitro system consisting of human glioma spheroids. Spheroids (350-400 μm dia.) were irradiated with 670 nm laser light in an incubator at temperatures ranging from 37 to 50 °C. For each temperature investigated (45 min. heating time), spheroids were divided into 5 groups: control, dark control, bleomycin-only, photodynamic therapy (PDT), and PCI. PDT and PCI spheroids were exposed to radiant exposures ranging from 0.5-3.0 J cm-2 using an irradiance of 5 mW cm-2. Toxicity was evaluated from spheroid growth kinetics. The combination of PCI and HT resulted in growth delays over a very narrow range of radiant exposures (1.5 – 2.5 J cm-2) and temperatures (40 – 42 °C).

Paper Details

Date Published: 8 March 2013
PDF: 8 pages
Proc. SPIE 8565, Photonic Therapeutics and Diagnostics IX, 85655F (8 March 2013); doi: 10.1117/12.2005407
Show Author Affiliations
Steen J. Madsen, Univ. of Nevada, Las Vegas (United States)
Christina Schlazer, Univ. of Nevada, Las Vegas (United States)
Aaron Andersen, Univ. of Nevada, Las Vegas (United States)
Stephanie Molina, Univ. of Nevada, Las Vegas (United States)
Henry Hirschberg, Beckman Laser Institute, Univ. of California, Irvine (United States)

Published in SPIE Proceedings Vol. 8565:
Photonic Therapeutics and Diagnostics IX
Andreas Mandelis; Brian Jet-Fei Wong; Anita Mahadevan-Jansen; Henry Hirschberg M.D.; Hyun Wook Kang; Nikiforos Kollias; Melissa J. Suter; Kenton W. Gregory M.D.; Guillermo J. Tearney M.D.; Stephen Lam; Bernard Choi; Steen J. Madsen; Bodo E. Knudsen M.D.; E. Duco Jansen; Justus F. Ilgner M.D.; Haishan Zeng; Matthew Brenner; Laura Marcu, Editor(s)

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