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Abstracts submitted after 22 December will be considered for poster presentation format only

Biophotonics as a research area provides and employs novel photonic technologies and tools for medical research, diagnosis and therapy. Biophotonics research aims for a deeper understanding of the processes within living cells, which is, on the other hand, a prerequisite for the improvement of early recognition and targeted treatment of diseases. The corresponding technological solutions and methods for diagnosis and therapy will be employed for an efficient and affordable health care which will help to deal with the challenges of aging societies and exploding health care costs. Resultant biophotonics systems will support not only the preservation, but even the increase of the impressive double-digit annual growth rates of the related industries. The development of such systems will require highly interdisciplinary collaboration based on an intensive dialogue between scientists from the various disciplines in order to streamline common efforts. An important part of biophotonic systems is based on spectroscopic, microscopic and imaging methods, not only for diagnostic purposes but also for surgical instruments, pathology applications and therapy control. Multimodal systems, based on combinations of different, not necessarily only photonic techniques will help to overcome the limits of the individual methods in combination with powerful deep learning and artificial intelligence based image analysis and chemometric methods.

Contributed papers are solicited concerning, but not limited to, the following areas:

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In progress – view active session
Conference 13006

Biomedical Spectroscopy, Microscopy, and Imaging III

8 - 11 April 2024 | Etoile B, Niveau/Level 1
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  • Hot Topics I
  • 1: Vibrational Spectroscopy and Imaging I
  • 2: Vibrational Spectroscopy and Imaging II
  • 3: Vibrational Spectroscopy and Imaging III
  • 4: Vibrational Spectroscopy and Imaging IV
  • 5: Advanced Imaging and Spectroscopy I
  • 6: Photoacoustics
  • Hot Topics II
  • Posters-Tuesday
  • 7: Optical Coherence Tomography I
  • 8: Optical Coherence Tomography II
  • 9: Optical Coherence Tomography III
  • 10: Fluorescence Imaging
  • 11: Advanced Imaging and Spectroscopy II
  • Hot Topics III
  • 12: Advanced Imaging and Spectroscopy III
  • 13: Advanced Imaging and Spectroscopy IV
  • 14: Microscopy
Hot Topics I
8 April 2024 • 09:00 - 11:00 CEST | Auditorium Schweitzer, Niveau/Level 0
Session Moderators:
Paul Montgomery, Univ. of Strasbourg (France)
2024 Symposium Chair

9:00 hrs: Welcome and Opening Remarks

9:10 hrs: City of Strasbourg Welcome

9:15 hrs: Speaker Introduction
12993-500
Author(s): Stefanie Barz, Univ. Stuttgart (Germany)
8 April 2024 • 09:20 - 10:05 CEST | Auditorium Schweitzer, Niveau/Level 0
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I will explore various facets of photonic quantum systems and their application in photonic quantum technologies. Firstly, I will focus into quantum foundations and by discuss quantum interference, a key element in photonic quantum technologies. I will highlight how the distinguishability and mixedness of quantum states influence the interference of multiple single photons – and demonstrate novel schemes for generating multipartite entangled quantum states. I will then address photonic quantum computing, specifically focusing on the building blocks of photonic quantum computers. This includes the generation of resource states essential for photonic quantum computing. I will then shift to photonic quantum networks, covering both their hardware aspects and showcasing quantum-network applications that extend beyond bi-partite quantum communication. Lastly, I will outline how photonic integration facilitates the scalability of these systems and discuss the associated challenges.
13013-501
Author(s): Malte C. Gather, Univ. zu Kolnn (Germany)
8 April 2024 • 10:10 - 10:55 CEST | Auditorium Schweitzer, Niveau/Level 0
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Joining the rich photophysics of organic light-emitting materials with the exquisite sensitivity of optical resonances to geometry and refractive index enables a plethora of devices with unusual and exciting properties. Examples from my team include biointegrated microlasers for real time sensing of cellular activity and long-term cell tracking, as well as the development of photonic implants with extreme form factors and wireless power supply that support thousands of individually addressable organic LEDs and thus allow optogenetic targeting of neurons deep in the brain with unprecedented spatial control. Very recently, by driving the interaction between excited states in organic materials and resonances in thin optical cavities into the strong coupling regime, we unlocked new tuning parameters which may play a crucial role in the next generation of TVs and computer displays to achieve even more saturated colour while retaining angle-independent emission characteristics.
Session 1: Vibrational Spectroscopy and Imaging I
8 April 2024 • 11:20 - 12:30 CEST | Etoile B, Niveau/Level 1
Session Chair: Jürgen Popp, Leibniz-Institut für Photonische Technologien e.V. (Germany)
13006-1
Author(s): Tamiki Komatsuzaki, Hokkaido Univ. (Japan)
8 April 2024 • 11:20 - 11:50 CEST | Etoile B, Niveau/Level 1
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We present our recent studies on on-the-fly Raman microscopy combined with multi-armed Bandits algorithm in reinforcement learning with spontaneous Raman measurements. This enables an several order acceleration of the measurements by designing and generating optimal illumination pattern “on the fly” during the measurements while keeping the accuracy of the discrimination. We will present the principle of the new microscopy and recent progress.
13006-2
Author(s): Margot Vandermotten, Tatevik Chalyan, Wendy Meulebroeck, VUB B-PHOTONICS, Vrije Univ. Brussel (Belgium); Leo A. van Grunsven, Vrije Univ. Brussel (Belgium); Heidi Ottevaere, VUB B-PHOTONICS, Vrije Univ. Brussel (Belgium)
8 April 2024 • 11:50 - 12:10 CEST | Etoile B, Niveau/Level 1
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The current methodology used to diagnose drug-induced liver injury (DILI) is rather exhaustive and complex. Using non-destructive Raman spectroscopy for high-content liver cell screening may offer an improved system for early DILI detection. This study explores the potential of Raman spectroscopy in tracking the progression of drug-induced liver fibrosis by monitoring the hepatic stellate cell (HSC) activation. We observed that the loss of retinoid content during HSC activation is accompanied by Raman spectral changes, allowing for discrimination between quiescent and activated HSCs based on their Raman data. This suggests that Raman spectroscopy can be ideal in drug-induced liver fibrosis detection during the drug development process.
13006-3
Author(s): Sara Abbasi, Mehdi Feizpour, Vrije Univ. Brussel (Belgium); Ilse Weets, Univ. Ziekenhuis Brussel (Belgium); Qing Liu, Hugo Thienpont, Francesco Ferranti, Heidi Ottevaere, Vrije Univ. Brussel (Belgium), Flanders Make (Belgium)
8 April 2024 • 12:10 - 12:30 CEST | Etoile B, Niveau/Level 1
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Our research investigates the potential of Raman spectroscopy combined with machine learning to enhance hemoglobinopathy screening. Hemoglobinopathies are common inherited diseases with serious health consequences. We use Raman spectroscopy to analyze hemoglobin fractions, enabling an accurate identification. By fine-tuning support vector machines (SVM) and fully connected neural networks (NN), we achieve test accuracies of 98.2% and 98.5%, respectively. Our research highlights the potential of Raman spectroscopy as an identification tool when combined with high-performance liquid chromatography. Furthermore, this detection approach can be easily miniaturized and integrated into microfluidics lab-on-chips.
Session 2: Vibrational Spectroscopy and Imaging II
8 April 2024 • 13:30 - 15:20 CEST | Etoile B, Niveau/Level 1
Session Chair: Tamiki Komatsuzaki, Hokkaido Univ. (Japan)
13006-4
Author(s): Joseph Chaiken, Syracuse Univ. (United States); Bin Deng, Athinoula A. Martinos Ctr. for Biomedical Imaging (United States); Paul W. Dent, Syracuse Univ. (United States); Isabel Szablewski, Columbia Univ. (United States); Sriram Narsipur, SUNY Upstate Medical Univ. (United States); Charles Peterson, Syracuse Univ. (United States)
8 April 2024 • 13:30 - 14:00 CEST | Etoile B, Niveau/Level 1
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Probing volar side fingertip capillary beds with 830 nm light produces remission spectra containing Rayleigh and Raman scattered light, and fluorescence, allowing continuous monitoring of intravascular plasma volume and hematocrit using the FRD-PVOH algorithm. During dialysis, Raman emission by polyatomic electrolytes i.e., phosphate and sulfate tracks with fluid removal i.e., the change in intravascular plasma volume, measured in vivo with FRD-PVOH and in agreement with simultaneous hematocrit measurement of extracorporeal blood in the dialysis unit using the FDA approved CritLine. The variation of Raman features associated with urea is more complicated, not exactly tracking the removal of electrolytes and water. Consistent with skin conditions induced by chronic kidney disease and dialysis, routine excess urea possibly destabilizes hydrogen bonding networks, exposing disulfide linkages, making them vulnerable to reduction by other species in the interstitial fluid. The various compartments in the human body do not drain at equal rates during dialysis. Real time spectroscopy-based feedback during hemodialysis may reduce the adverse events, improving outcomes.
13006-5
Author(s): Tigran Mansuryan, XLIM, Univ. de Limoges (France); Alessandro Tonello, XLIM (France); Katarzyna Krupa, Institute of Physical Chemistry (Poland); Alexis Desmoulière, Mark Arthur Ndong Ntoutoume, Vincent Sol, Univ. de Limoges (France); Claire Lefort, XLIM, Univ. de Limoges (France); Mario Zitelli, Mario Ferraro, Sapienza Univ. di Roma (Italy); Fabio Mangini, Univ. degli Studi di Brescia (Italy); Yifan Sun, Sapienza Univ. di Roma (Italy); Yago Arosa, XLIM (France), Univ. de Santiago de Compostela (Spain); Benjamin Wetzel, XLIM (France); Stefan Wabnitz, Sapienza Univ. di Roma (Italy), Istituto Nazionale di Ottica, Consiglio Nazionale delle Ricerche (Italy); Vincent Couderc, XLIM (France)
8 April 2024 • 14:00 - 14:20 CEST | Etoile B, Niveau/Level 1
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Multiplex Coherent Anti-Stokes Raman Scattering (M-CARS) is an innovative nonlinear spectroscopic approach designed to characterize the vibrational modes of molecular structures. Coherent Raman scattering has been used for the characterization of biomedical targets for about 20 years and the multiplex aspect was introduced 10 years ago thanks to the use of a supercontinuum laser system. For each of these systems, the combination of a pump and a probe wave, driven by an external delay line, is however required to produce the vibrations. In the present work, we propose a new M-CARS system, free of the external delay line. A few-mode microstructured fiber enables merging both wave-packets (pump and supercontinuum) within a single waveguide. We showcase the capability of this system in generating hyperspectral images of biochemically active compounds. Curcumin I, the principal yellow compound isolated from Curcuma longa (Turmeric), is distinguishable by its multiple functional groups that display a nonlinear spectroscopic behavior.
13006-6
Author(s): Hulya Yilmaz, Anuradha Ramoji, Leibniz-Institut für Photonische Technologien e.V. (Germany), Abbe Ctr. of Photonics, Friedrich-Schiller-Univ. Jena (Germany); Anja Silge, Leibniz-Institut für Photonische Technologien e.V. (Germany), Abbe Ctr. of Photonics, Friedrich-Schiller-Univ. Jena (Germany), Forschungcampus InfectoGnostics (Germany); Aikaterini Pistiki, Leibniz-Institut für Photonische Technologien e.V. (Germany); Karina Weber, Oleg Ryabchykov, Leibniz-Institut für Photonische Technologien e.V. (Germany), Forschungcampus InfectoGnostics (Germany); David Vasquez Pinzon, Iwan W. Schie, Leibniz-Institut für Photonische Technologien e.V. (Germany); Franziska Hornung, Stefanie Deinhardt-Emmer, Bettina Löffler, Universitätsklinikum Jena (Germany); Jürgen Popp, Leibniz-Institut für Photonische Technologien e.V. (Germany), Abbe Ctr. of Photonics, Friedrich-Schiller-Univ. Jena (Germany), Forschungcampus InfectoGnostics (Germany)
8 April 2024 • 14:20 - 14:40 CEST | Etoile B, Niveau/Level 1
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High-throughput screening Raman Spectroscopy (HTS-RS) is a breakthrough tool for clinical applications as it helps detect infection-associated cell activation. The technique captures the scattered light as a spectrum, presenting the sample's molecular signature. Machine learning methods are used to analyze the data confidently. Our study examined the influence of the interaction time between the virus and the cell on the spectral phenotype. HTS-RS has the potential to phenotype and identify cell activation associated with infections. This research could prove pivotal in developing effective strategies for diagnosing and treating the virus. Our findings provide a new information dimension of the cellular host response to virus infection.
13006-7
Author(s): Mehrdad Lotfi Choobbari, Vrije Univ. Brussel (Belgium)
8 April 2024 • 14:40 - 15:00 CEST | Etoile B, Niveau/Level 1
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Inappropriate management of plastic waste together with the large demand for plastic products endangered our environment with the problem of microplastic (MP) pollution. Microplastics may be released from blended polymers with complex structures making their analysis challenging. Here, we showcase the application of 3-D micro-Raman spectroscopy as a promising approach for the accurate study of blended microplastics (B-MPs). Moreover, the application of a line-shaped laser line focus is introduced to shorten the measurement time of 3-D Raman mapping from 56 to 2 hours, still acquiring valuable information about the morphology and concentration of polymers in B-MPs.
13006-8
Author(s): Liam Collard, Mohammadrahim Kazemzadeh, Linda Piscopo, Filippo Pisano, Massimo De Vittorio, Ferruccio Pisanello, Istituto Italiano di Tecnologia (Italy)
8 April 2024 • 15:00 - 15:20 CEST | Etoile B, Niveau/Level 1
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Raman spectroscopy is a powerful technique used across the life sciences to measure the molecular composition of a sample. There has been growing interest to miniaturize Raman imaging devices for endoscopic applications, however typically these probes are based on fiber bundles which increase the overall footprint of the probe. Recent works have shown that by applying a wavefront shaping technique, a single fiber may be transformed into a sub-cellular resolution Raman endoscope. However, a single probe both exciting and collecting the signal leads to an unavoidable large background signal from the fiber itself, masking large portions of the Raman signal from the sample. Here, we adopt a data-driven approach to de-convolve the background signal from the sample. In particular, we demonstrate that by applying PCA and machine learning techniques, sub-cellular resolution Raman images of pharmaceutical clusters can be made with supervision-free analysis.
Session 3: Vibrational Spectroscopy and Imaging III
8 April 2024 • 15:40 - 18:10 CEST | Etoile B, Niveau/Level 1
Session Chair: Joseph Chaiken, Syracuse Univ. (United States)
13006-9
Author(s): Hongje Jang, Zhi Li, Yajuan Li, Yu Ping, Lingyan Shi, Univ. of California, San Diego (United States)
8 April 2024 • 15:40 - 16:10 CEST | Etoile B, Niveau/Level 1
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We've established a nonlinear multimodal imaging system that incorporates stimulated Raman Scattering (SRS), multiphoton fluorescence (MPF), and second harmonic generation (SHG) to explore the connections between metabolic activities and the distribution of metabolites in cells and tissues. Furthermore, we've devised the Adam-based Pointillism Deconvolution (A-PoD) and Correlation Coefficient Mapping (CoCoMap) algorithms, enabling a deeper insight into the simultaneous recording and regulation of various metabolic processes within super-resolved images of nanoscale Regions of Interest (ROIs). In our pursuit of specifically identifying signals originating from distinct subcellular organelles, we've introduced a pioneering clustering algorithm known as Multi-SRS reference matching (Multi-SRM). This approach has the potential to improve early disease detection, prognosis, the evaluation of therapeutic effects, and our comprehension of the mechanisms underpinning aging and biomedicine.
13006-10
Author(s): Bryce Manifold, Bowen Yang, Denis Titov, Aaron Streets, Univ. of California, Berkeley (United States)
8 April 2024 • 16:10 - 16:30 CEST | Etoile B, Niveau/Level 1
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Aging related biological mechanisms are often difficult to probe in situ without exogenous fluorophores. Here, we leverage label-free stimulated Raman scattering (SRS) microscopy to provide new insights into aging in C. elegans. We demonstrate multispectral SRS imaging of whole worms in vivo with quantitative chemical insights across different ages. We show that both lipid and protein synthesis and compartmentalization are associated with aging in worms. We additionally use SRS in combination with simultaneous two-photon fluorescence imaging to characterize the putatively aberrant protein accumulation. Moreover, we observe notable SRS image differences when worms are subjected to calorie restriction, suggesting a promising avenue towards understanding calorie-restriction’s enhancing effects on longevity when coupled to proteomic and metabolomic analysis.
13006-11
Author(s): Hossein Khadem, Istituto per l'endocrinologia e l'oncologica sperimentale "Gaetano Salvatore", Consiglio Nazionale delle Ricerche (Italy); Maria Antonietta Ferrara, Istituto di Scienze Applicate e Sistemi Intelligenti "Eduardo Caianiello", Consiglio Nazionale delle Ricerche (Italy); Maria Mangini, Alberto Luini, Istituto per l'endocrinologia e l'oncologica sperimentale "Gaetano Salvatore", Consiglio Nazionale delle Ricerche (Italy); Giuseppe Coppola, Istituto di Scienze Applicate e Sistemi Intelligenti "Eduardo Caianiello", Consiglio Nazionale delle Ricerche (Italy); Anna Chiara De Luca, Istituto per l'endocrinologia e l'oncologica sperimentale "Gaetano Salvatore", Consiglio Nazionale delle Ricerche (Italy)
8 April 2024 • 16:30 - 16:50 CEST | Etoile B, Niveau/Level 1
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In this study, we utilized holotomography to analyze prostate healthy (PNT2) and cancer (PC3) cells treated with glucose. After 48-hour incubation, distinct morphological differences were observed in cancer cells, including changes in lipid droplet volume, number, and refractive index. Raman spectroscopy confirmed the presence of these lipid droplets. Cancer cells exhibited larger and more numerous lipid droplets, with higher mean refractive-index, compared to healthy cells. The study achieved over 90% accuracy in discriminating between cell types, highlighting its potential in cancer diagnostics. The research contributes to biomedical spectroscopy, offering a valuable tool for understanding cancer cell morphology and enabling early and precise cancer diagnosis.
13006-12
Author(s): Katharina Frings, Leibniz Univ. Hannover (Germany); Nils Heine, Medizinische Hochschule Hannover (Germany); Nicolas Debener, Thomas Scheper, Leibniz Univ. Hannover (Germany); Janina Bahnemann, Univ. Augsburg (Germany); Katharina Nikutta, Medizinische Hochschule Hannover (Germany); Maria Leilani Torres-Mapa, Alexander Heisterkamp, Leibniz Univ. Hannover (Germany)
8 April 2024 • 16:50 - 17:10 CEST | Etoile B, Niveau/Level 1
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Implant associated infections can result in inflammatory conditions such as periimplantitis, that can ultimately lead to implant loss. This is caused by a large community of bacterial species. These bacteria form a multispecies biofilm on the tooth surface. Its composition can shift as it matures over time, the so-called pathogenic shift occurs once pathogens adhere to the biofilm. We developed a measurement protocol and analysis pipeline based on ATR-FTIR spectroscopy that is able to distinguish between different oral bacteria by detecting slight changes in protein expression. This can easily be done for single species samples with supervised learning algorithms like the k-nearest neighbour algorithm, with which we achieved a prediction accuracy of 99.8 %. Chemometric and deep learning approaches can streamline the process in distinguishing multispecies samples. This would be a step towards early detection of the pathogenic shift in oral biofilms and help avoiding diseases.
13006-13
Author(s): Hiroki Cook, Anna Crisford, Univ. of Southampton (United Kingdom); Jitendra Nath Acharyya, Institut d'Optique Graduate School, Univ. de Bordeaux (France), Indian Institute of Technology Delhi (India); Konstantinos N. Bourdakos, Janos Kanczler, Douglas Dunlop, Richard O. C. Oreffo, Sumeet Mahajan, Univ. of Southampton (United Kingdom)
8 April 2024 • 17:10 - 17:30 CEST | Etoile B, Niveau/Level 1
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Osteoarthritis (OA), a degenerative joint disease presenting as loss of cartilage, is a leading cause of disability worldwide, increasingly with aging populations. Early detection is crucial for effective treatment since there is no definitive cure, yet, current assessment techniques fall short and rely on ionising radiation or invasive procedures. We report an application of multimodal “spectromics”, low-level abstraction data fusion of non-destructive NIR Raman and NIR-SWIR absorption spectroscopy, providing an enhanced, interpretable “fingerprint” for diagnosis of OA in human cartilage. Under multivariate statistical analyses and supervised machine learning, cartilage was classified with high precision and disease state predicted accurately. Discriminatory features within the spectromics fingerprint elucidated clinically relevant tissue components (OA biomarkers). Further, we have developed an automated goniometric 3D hyperspectral mapping setup, and characterised OA cartilage on whole human femoral heads post hip arthroplasty for spatially-resolved spectromics. These results lay foundation for minimally invasive, deeply penetrating, label-free, chemometric diagnosis of the hip.
13006-14
Author(s): Sara Stefani, Paola Sassi, Univ. degli Studi di Perugia (Italy); Alessandro Cataliotti, Reza Parvan, Institute for Experimental Medical Research, Oslo Univ. Hospital (Norway); Martina Alunni Cardinali, Marco Paolantoni, Univ. degli Studi di Perugia (Italy); Gustavo Jose Justo da Silva, Institute for Experimental Medical Research, Oslo Univ. Hospital (Norway)
8 April 2024 • 17:30 - 17:50 CEST | Etoile B, Niveau/Level 1
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Clinical evidence underscores the intricate interplay between the heart and kidneys, where dysfunction in one organ contributes to progressive failure of both. Using vibrational spectroscopy techniques, we demonstrated molecular changes in cardiac tissue post-uninephrectomy and ischemia-reperfusion in a rat cardiorenal syndrome (CS) model. It is now imperative to investigate whether structural changes in renal tissue following these two interventions are detectable. In response to the lack of renal-focused pharmacology for cardiorenal syndrome (CS), we also investigated the efficacy of the drug ProANP31-67, a peptide targeting cardiorenal complications that has already shown improvement in cardiac conditions following both pathologies. Micro-FTIR and micro-Raman spectroscopy were used to analyze kidney tissues, employing a dedicated R programming protocol to facilitate efficient analysis.
13006-15
Author(s): Sebastian Huelck, tec5USA, Inc. (United States)
8 April 2024 • 17:50 - 18:10 CEST | Etoile B, Niveau/Level 1
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Amid the SARS-CoV-2 pandemic, traditional virus detection methods like RT-qPCR face limitations in terms of infrastructure and processing time. This has spurred the development of agile diagnostic technologies, emphasizing non-invasive and rapid testing. Surface-Enhanced Raman Scattering (SERS) and Localized Surface Plasmon Resonance (LSPR) have emerged as promising alternatives. SERS, amplifying Raman signals through metal nanostructures, offers high sensitivity, high specificity, rapid response, qualitative and quantitative analysis enhanced by recent innovations like multiwell-array substrates. Integration with machine learning refines SERS's diagnostic capabilities, enabling rapid and accurate identification of SARS-CoV-2. LSPR, leveraging light-metal nanoparticle interactions, revolutionizes rapid viral detection, especially with the development of portable handheld devices. These devices enable real-time, on-site testing, proving crucial in managing infectious disease outbreaks. Their applications extend beyond SARS-CoV-2, holding potential for various pathogens.
Session 4: Vibrational Spectroscopy and Imaging IV
9 April 2024 • 08:30 - 10:30 CEST | Etoile B, Niveau/Level 1
Session Chair: Hongje Jang, Univ. of California, San Diego (United States)
13006-16
Author(s): Paola Sassi, Univ. degli Studi di Perugia (Italy)
9 April 2024 • 08:30 - 09:00 CEST | Etoile B, Niveau/Level 1
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Heart Failure with Preserved Ejection Fraction (HFpEF) is a severe medical condition. Concurrent pathologies linked with HFpEF create a complex scenario that contributes to structural and functional abnormalities in the heart and kidneys—the principal end organs affected by HFpEF. In this study, we assessed the effectiveness of a synergistic application of FTIR and Raman spectroscopy and multivariate/univariate statistical analyses to yield valuable pathophysiological insights in a rat model of heart failure. Peculiar biochemical differences were detected in the two organs demonstrating heightened sensitivity of these techniques towards the distinctive HFpEF phenotype.
13006-17
Author(s): Martina Alunni Cardinali, Univ. degli Studi di Perugia (Italy); Marco Govoni, Reconstructive Orthopaedic Surgery and Innovative Techniques – Musculoskeletal Tissue Bank, IRCCS (Italy); Sara Stefani, Univ. degli Studi di Perugia (Italy); Alessandra Maso, IRCCS, Istituto Ortopedico Rizzoli (Italy); Elisa Storni, IRCSS, Istituto Ortopedico Rizzoli (Italy); Dante Dallari, Reconstructive Orthopaedic Surgery and Innovative Techniques – Musculoskeletal Tissue Bank, IRCCS (Italy); Daniele Fioretto, Paola Sassi, Univ. degli Studi di Perugia (Italy)
9 April 2024 • 09:00 - 09:20 CEST | Etoile B, Niveau/Level 1
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Staphylococcus aureus (SA) is the most commonly isolated aetiological agent of osteomyelitis and periprosthetic joint infection (PJI), a major public health problem in western countries. Infections usually result from bacterial spread through fractures or implants and their treatment requires removal of infected tissue, thorough cleansing of the wound and administration of antibiotics directly to the site of infection. Despite a precise surgical procedure, removal and replacement of the medical device is often necessary when an infected prosthesis is involved. Here we discuss a methodology for investigating the impact of SA infection on the structure and chemical composition of bone tissue, based on the integration of Raman-Brillouin microspectroscopy (BRamS) and AFT-FTIR spectroscopy. Our aim is to improve the understanding of the effects of SA infection on bone tissue and to identify specific markers that can be used for the detection of damaged tissue.
13006-18
Author(s): Keertana Vinod Ram, U.S. Dinish, A*STAR Skin Research Labs., A*STAR Agency for Science, Technology and Research (Singapore); Yik Weng Yew, National Skin Ctr. Pte. Ltd. (Singapore); Renzhe Bi, A*STAR Skin Research Labs., A*STAR Agency for Science, Technology and Research (Singapore); Amalina Binte Ebrahim Attia, Biomedical Research Council, A*STAR Agency for Science, Technology and Research (Singapore); Valerie Teo Xinhui, Poongkulali Rajarahm, A*STAR Skin Research Labs., A*STAR Agency for Science, Technology and Research (Singapore); Hazel H. Oon, National Skin Ctr. Pte. Ltd. (Singapore), A*STAR Agency for Science, Technology and Research (Singapore); Steven Tien Guan Thng, National Skin Ctr. Pte. Ltd. (Singapore); Malini C. Olivo, A*STAR Skin Research Labs., A*STAR Agency for Science, Technology and Research (Singapore)
9 April 2024 • 09:20 - 09:40 CEST | Etoile B, Niveau/Level 1
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Atopic dermatitis (AD) and psoriasis are the two most prevalent skin disorders, often assessed through subjective questionnaires or visual evaluations conducted by clinicians, which can be subject to interpersonal variations. This study aims to explore the distinctions between these skin conditions and healthy skin using a portable confocal Raman spectroscopy (CRS) system for objective assessment. Spectral measurements at 671 nm and 785 nm on 9 AD, 6 psoriasis, and 11 healthy subjects reveal lower water content in AD compared to psoriasis and healthy skin. Ceramide subclasses show disease-specific trends, distinguishing AD, and psoriasis. Cholesterol levels further differentiate these conditions, with lower concentrations in lesional AD and significantly higher concentrations in lesional psoriasis compared to healthy skin. These differences contribute to the objective differentiation of skin conditions aiding in thorough assessment and treatment monitoring. Furthermore, it offers valuable insights for developing targeted disease-specific topical treatments.
13006-19
Author(s): Maximilian Brinkmann, Maryam Rezaei, Ramon Droop, Refined Laser Systems GmbH (Germany); Kai M. Eder, Biomedizinisches Technologiezentrum (Germany); Felix Neumann, Refined Laser Systems GmbH (Germany); Björn Kemper, Biomedizinisches Technologiezentrum (Germany); Christoph Engwer, Refined Laser Systems GmbH (Germany); Jürgen Schnekenburger, Biomedizinisches Technologiezentrum (Germany); Tim Hellwig, Refined Laser Systems GmbH (Germany)
9 April 2024 • 09:40 - 10:00 CEST | Etoile B, Niveau/Level 1
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We present a compact and portable stimulated Raman scattering (SRS) imaging system capable of high speed, label-free imaging of cells and tissues. Our setup rapidly acquires multispectral datasets with high chemical specificity by scanning the spectral range of 700-3100 1/cm in just 100 ms, providing a tenfold increase in acquisition speed. It allowed to visualize cell nucleus and cytoplasm changes during drug induced cancer cell death. SRS offers distinct advantages, providing valuable insights into drug-cell interactions, cell morphology, and chemical changes without the interference of exogenous labels. The novel high-speed SRS systems allows rapid screening of drug effects on cancer cells.
13006-20
Author(s): Clémence Gentner, Lab. Kastler Brossel (France); Samuel Burri, Edoardo Charbon, Claudio E. Bruschini, Ecole Polytechnique Fédérale de Lausanne (Switzerland); Hilton Barbosa de Aguiar, Lab. Kastler Brossel (France)
9 April 2024 • 10:00 - 10:30 CEST | Etoile B, Niveau/Level 1
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Compressive Raman imaging has emerged as a promising technique to speed up chemical imaging by compressing the data during acquisition. Yet, current scanning imaging speed is fundamentally limited by the sensors pixel dwell times of at best 1µs. Here, we introduce a compressive Raman spectrometer layout equipped with a novel parallelized spatial acquisition using a single-photon avalanche detector array. We show imaging with pixel dwell times of <10µs using the otherwise weak spontaneous Raman effect, thereby unlocking video-rate imaging.
Session 5: Advanced Imaging and Spectroscopy I
9 April 2024 • 10:50 - 13:00 CEST | Etoile B, Niveau/Level 1
Session Chair: Hilton Barbosa de Aguiar, Lab. Kastler Brossel (France)
13006-21
Author(s): Artjoms Suponenkovs, Dmitrijs Bliznuks, Riga Technical Univ. (Latvia); Alexey Lihachev, Univ. of Latvia (Latvia); Roberts Tarvids, Loughborough Univ. (United Kingdom)
9 April 2024 • 10:50 - 11:20 CEST | Etoile B, Niveau/Level 1
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The problem of automated bacterial colony counting is a very relevant one, due to the high importance of bacteriological analysis. Moreover, this automated counting saves biologists time and improves the accuracy of their experiments. This paper has two aims: to investigate the challenges of automated bacterial colony counting, and to address the joint challenges of petri dish localization and bacterial colony reflections in such dishes. These reflections can seriously reduce the accuracy of automated bacterial colony counting. Therefore, the main aim of this paper is to show new methods for detecting and removing bacterial colony reflections in a petri dish by the use of computer vision. The experimental part of the paper contains the results, and descriptions of petri dish localization, and detecting and removing bacterial colony reflections. The proposed methods and the data obtained from these experiments significantly improve the accuracy of automated bacterial colony counting.
13006-22
Author(s): Marc Grosjean, Antoine Delon, Irene Wang, Lab. Interdisciplinaire de Physique (France); Olivier Destaing, Alexei Grichine, Mylène Pezet, Institut pour l'Avancée des Biosciences (France)
9 April 2024 • 11:20 - 11:40 CEST | Etoile B, Niveau/Level 1
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The possibility to precisely tune the spatial and temporal properties of proteins activity is the major promise of optogenetics. This would require a precise control in 3D of the activating light distribution, which is not easily achieved. Today, light can be patterned with micrometric accuracy, but only in a plane transverse to its propagation axis, while it remains unconstrained along this axis. In contrast, total internal reflection fluorescence (TIRF) microscopy uses an evanescent field to confine light in depth, but does not provide transverse patterning in the sample plane. Here, we present a method to combine transverse and axial illumination confinement: evanescent wave patterning. Our approach is based on wavefront-shaping using a digital micro-mirror device (DMD). We show multiple evanescent patterns on a fluorescent solution and on cells transfected with an optogenetics system.
13006-23
Author(s): Hamed Abbasi, Technische Univ. Delft (Netherlands), Erasmus MC (Netherlands); Loes Ettema, Technische Univ. Delft (Netherlands); Michail Doukas, Sjors A. Koppes, Stijn Keereweer, Erasmus MC (Netherlands); Miriam Menzel, Technische Univ. Delft (Netherlands)
9 April 2024 • 11:40 - 12:00 CEST | Etoile B, Niveau/Level 1
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Studying fiber architectures in tumor tissues is of great importance as the cancer cells interact with fiber structures in the Extracellular Matrix (ECM). Computational Scattered Light Imaging (ComSLI) represents an innovative, non-destructive approach to whole-slide imaging with micrometer resolution, uniquely capable of accurately unraveling the complex, intertwined fiber structures in biological tissues. So far, it has been mainly used to study highly interwoven nerve fiber architectures in brain tissues. In this study, we extend the application of ComSLI to visualize fibers in diverse tumor tissues, including oral squamous cell carcinoma (OSCC) and colorectal cancer.
13006-24
Author(s): Remy Avila, Melissa Matrecitos-Avila, Reinher Pimentel-Domínguez, Univ. Nacional Autónoma de México (Mexico); Elisa Tamariz, Univ. Veracruzana (Mexico); Pablo Loza-Alvarez, ICFO - Institut de Ciències Fotòniques (Spain); Edén J. Parra-Fuentes, Univ. Nacional Autónoma de México (Mexico)
9 April 2024 • 12:00 - 12:20 CEST | Etoile B, Niveau/Level 1
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Precision and stability in confocal microscopy are vital for maintaining image clarity and preventing misinterpretation of radiometric fluctuations as changes in fluorescence intensity within the sample. This study discusses a focus variation induced by an 810-nm continuous wave laser integrated into the optical path of an inverted confocal microscope with a 60x oil immersion objective. Activation or deactivation of the laser leads to a drift in the focus position towards lower or higher values of the vertical coordinate z, respectively. The maximum observed drift is 2.25 μm, occurring with a 40 mW laser power at the sample over a 600-second exposure time. The temporal behavior of the focus position follows exponential curves resembling temperature fluctuations associated with a heat source. Our analysis strongly indicates that the focus drift is attributable to the heating of the immersion oil.
13006-25
Author(s): Vijay Prabhakar, Sunita Bhatt, Satish K. Dubey, Indian Institute of Technology Delhi (India)
9 April 2024 • 12:20 - 12:40 CEST | Etoile B, Niveau/Level 1
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Using machine learning techniques like deep neural networks for classifying plant diseases in images. Manual classification of plant diseases is time-consuming and requires trained professionals. Early detection of plant diseases is important for food security. Transfer learning with pre-trained neural networks is an effective approach for classifying plant diseases in images, with the potential to help scale up disease detection efforts.
13006-26
Author(s): Ruipeng Wang, Yu Cai, Yaowei Liu, Qili Zhao, Mingzhu Sun, Xin Zhao, Yue Du, Nankai Univ. (China)
9 April 2024 • 12:40 - 13:00 CEST | Etoile B, Niveau/Level 1
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This study explored the relationship between the structure similarity coefficient extracted from time-lapse monitoring videos of embryos and the embryonic developmental process. It applied the K-means clustering method to analyze the clustering of structure similarity coefficients of different samples, and to investigate the correlation between the clusters with the ability of embryonic cells to develop into blastocysts. This study generated an effective predictive marker for morphological changes in embryonic cell development, contributing to the prediction of the developmental potential of embryonic cells.
Session 6: Photoacoustics
9 April 2024 • 14:00 - 16:10 CEST | Etoile B, Niveau/Level 1
Session Chair: Gregory Baethge, Ansys Canada Ltd. (United Kingdom)
13006-27
Author(s): Robert Nuster, Guenther Paltauf, Karl-Franzens-Univ. Graz (Austria)
9 April 2024 • 14:00 - 14:30 CEST | Etoile B, Niveau/Level 1
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A promising alternative ultrasound detection scheme in photoacoustic imaging is the use of an optical camera to achieve massively parallelized data acquisition. For this purpose, the millions of pixels of common CMOS- or CCD-cameras are used to capture ultrasound-induced intensity modulations of a light field. Depending on the interaction mechanism in the propagation medium or at an interface, either projections or sectional images of the pressure field are recorded directly by means of free beam propagation or indirectly via optical fibers at certain times of wave propagation. Here we present the functionality of different experimental implementations and discusses their advantages and disadvantages in terms of achievable sensitivity, bandwidth, data structure, image acquisition rate, suitability for multimodal imaging, compactness of the implementation and gives an outlook on future new developments in this direction.
13006-28
Author(s): Ivana Falco, Guillaume Godefroy, Bastien Arnal, Emmanuel Bossy, Lab. Interdisciplinaire de Physique, Univ. Grenoble Alpes, CNRS (France)
9 April 2024 • 14:30 - 14:50 CEST | Etoile B, Niveau/Level 1
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Conventional photoacoustic (PA) imaging suffers from visibility artefacts due to limitations in ultrasound transducer bandwidth, viewing angles, and the use of sparse arrays. PA fluctuation imaging (PAFI), exploiting the signal changes due to blood flow, compensates for these artefacts, at the cost of temporal resolution. Our study addresses this limitation employing a deep learning approach in which PAFI images serve as ground truths for training a 3D neural network to obtain real-time single-shot artefact-free images. Following a pre-training with simulated examples, a 3D-ResUnet network was trained with 90 PA chicken embryo vasculature volumes as input and corresponding PAFI as ground truths. Notably, inclusion of experimental data significantly improves predictions over simulation-only training, even accounting for transducer angular filtering. Furthermore, applying the same network exclusively trained in-ovo to predict the femoral artery in mice demonstrates the potential of this method for real-time, full-visibility multispectral PA imaging in vivo using sparse arrays.
13006-29
Author(s): Jan Sievers, Martin-Luther-Univ. Halle-Wittenberg (Germany); Claus Villringer, Technische Hochschule Wildau (Germany); Werner Lebek, Taravat Gilani, Jan Laufer, Martin-Luther-Univ. Halle-Wittenberg (Germany)
9 April 2024 • 14:50 - 15:10 CEST | Etoile B, Niveau/Level 1
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Fabry-Perot tomographs have captured compelling photoacoustic images as they combine small element sizes with high acoustic sensitivity and a broad frequency response. A photoacoustic tomograph based on a sCMOS camera and a Fabry-Perot sensor with uniform optical thickness was developed. The influence of camera parameters on the e.g., spatial distribution of the acoustic sensitivity was evaluated. The imaging capabilities were demonstrated by capturing images of PA phantoms.
13006-30
Author(s): Marzieh Ezzatpour, Farzin Ghane Golmohamadi, Jan Laufer, Martin-Luther-Univ. Halle-Wittenberg (Germany)
9 April 2024 • 15:10 - 15:30 CEST | Etoile B, Niveau/Level 1
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The development of photoacoustic contrast agents relies on the recovery of the photophysical properties from cuvette measurements. Photoacoustic signals are typically described using the Beer-Lambert law. In contrast agents with long excited state lifetimes, it is no longer valid. This is particularly noticeable at low concentrations and short cuvette pathlengths as acoustic propagation and detection effects become significant. A forward model is used to calculate time-resolved signals generated in a cuvette. The model is fitted to measured signals to recover absorption coefficients. The model will enable the characterization of new contrast agents by recovering the spatial distribution of thermalized energy.
13006-31
Author(s): Guo Tang, Martin-Luther-Univ. Halle-Wittenberg (Germany); Jiaqi Zhu, Univ. College London (United Kingdom); Felix F. Lucka, Ctr. Wiskunde & Informatica (Netherlands); Teemu Sahlström, Univ. of Eastern Finland (Finland); Ben T. Cox, Univ. College London (United Kingdom); Jan Laufer, Martin-Luther-Univ. Halle-Wittenberg (Germany)
9 April 2024 • 15:30 - 15:50 CEST | Etoile B, Niveau/Level 1
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Three-dimensional quantitative photoacoustic tomography (3D-QPAT) aims to recover tissue chromophore concentrations from multispectral images but is often hampered by the unknown light fluence and the transfer function of the scanner. Inversion schemes that use hybrid light transport and acoustic propagation models may be used to address this challenge. While model-based inversions have shown promising results in in silico and tissue phantom studies, limitations in accuracy arose from limited view artefacts. This study evaluated reconstruction methods such as time-reversal, maximum a posteriori, iterative least square and total variation to improve the accuracy of 3D-QPAT inversion techniques.
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Author(s): Nagendra Singh, Shivam Rakhoulya, Samir K. Biswas, Indian Institute of Science Education and Research Mohali (India)
9 April 2024 • 15:50 - 16:10 CEST | Etoile B, Niveau/Level 1
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This work demonstrates the fabrication of high frequency ultrasound transducers, which is capable of sensing high frequency and broad bandwidth photoacoustic and ultrasound signals. For sensor film fabrication purposes, a piezoelectric composite polymer consisting of PVDF-TrFE (Polyvinylidene difluoride trifluoroethylene) and lab synthesized single crystal BSTO (Barium Strontium Titanate) nanofiller is developed. The piezoelectric efficiency after addition of BSTO is characterized using FTIR spectroscopy. The fabricated transducer is tested in the pulse echo mode and the central frequency is found to be 43 MHz with a bandwidth of 40 MHz (93% at -6dB). Photoacoustic signals of multiple frequencies are detected using the fabricated transducer. The high sensitivity and broad frequency spectrum of the transducer makes it a suitable candidate for high resolution photoacoustic and ultrasound imaging for biomedical imaging and nondestructive testing.
Hot Topics II
9 April 2024 • 16:30 - 18:05 CEST | Auditorium Schweitzer, Niveau/Level 0
Session Moderator:
Anna Mignani, Istituto di Fisica Applicata "Nello Carrara" (Italy)
2024 Symposium Chair

16:30 hrs
Welcome and Opening Remarks
Speaker Introduction
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Author(s): Kathy Lüdge, Technische Univ. Ilmenau (Germany)
9 April 2024 • 16:35 - 17:20 CEST | Auditorium Schweitzer, Niveau/Level 0
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Optical cavities with nonlinear elements and delayed self-coupling are widely explored candidates for photonic reservoir computing (RC). For time series prediction applications that appear in many real-world problems, energy efficiency, robustness and performance are key indicators. With this contribution I want to clarify the role of internal dynamic coupling and timescales on the performance of a photonic RC system and discuss routes for optimization. By numerically comparing various delay-based RC systems e.g., quantum-dot lasers, spin-VCSEL (vertically emitting semiconductor lasers), and semiconductor amplifiers regarding their performance on different time series prediction tasks, to messages are emphasized: First, a concise understanding of the nonlinear dynamic response (bifurcation structure) of the chosen dynamical system is necessary in order to use its full potential for RC and prevent operation with unsuitable parameters. Second, the input scheme (optical injection, current modulation etc.) crucially changes the outcome as it changes the direction of the perturbation and therewith the nonlinearity. The input can be further utilized to externally add a memory timescale that is needed for the chosen task and thus offers an easy tunability of RC systems.
12995-501
3D laser nanoprinting (Plenary Presentation)
Author(s): Martin Wegener, Karlsruher Institut für Technologie (Germany)
11 April 2024 • 17:20 - 18:05 CEST | Auditorium Schweitzer, Niveau/Level 0
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3D laser nanoprinting based on multi-photon absorption (or multi-step absorption) has become an established commercially available and widespread technology. Here, we focus on recent progress concerning increasing print speed, improving the accessible spatial resolution beyond the diffraction limit, increasing the palette of available materials, and reducing instrument cost.
Posters-Tuesday
9 April 2024 • 18:10 - 20:00 CEST | Galerie Schweitezer, Niveau/Level 0
Conference attendees are invited to attend the Photonics Europe poster session on Tuesday evening. Come view the posters, enjoy light refreshments, ask questions, and network with colleagues in your field. Authors of poster papers will be present to answer questions concerning their papers. Attendees are required to wear their conference registration badges to the poster sessions.

Poster Setup: Tuesday 10:00 - 17:30 hrs
Poster authors, view poster presentation guidelines and set-up instructions at http://spie.org/EPE/poster-presentation-guidelines.
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Author(s): Antreas Theodosiou, Lumoscribe Ltd. (Cyprus); Andreas Stylianou, European Research Ctr. Cyprus (Cyprus)
9 April 2024 • 18:10 - 20:00 CEST | Galerie Schweitezer, Niveau/Level 0
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In cancer research, accurate characterization of the tumor microenvironment (TME) is crucial for diagnosis and treatment. Desmoplasia, a cancer-specific fibrosis due to collagen overproduction, hinders treatment efficacy. This study introduces a fiber optic method utilizing adaptive focus light and fluorescence sensing to detect collagen in fresh cancer specimens. Conventional methods struggle with complex TME. Our approach, validated with diverse cancer samples, accurately identifies collagen. The method simplifies Atomic Force Microscopy, allowing multimodal characterization. This research revolutionizes cancer tissue analysis, providing a toolset for researchers and clinicians. Understanding collagen-cancer interplay opens avenues for targeted therapies, advancing cancer diagnostics and treatment.
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Author(s): Meital Harel, Ariel Univ. (Israel)
9 April 2024 • 18:10 - 20:00 CEST | Galerie Schweitezer, Niveau/Level 0
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Fluorescence lifetime imaging (FLI) has a major influence on our comprehension of biological systems, serving as a potent instrument for non-invasive exploration of biomolecular and cellular dynamics, both in vitro and in vivo. Its capacity to selectively target and multiplex diverse entities, coupled with heightened sensitivity and specificity, furnishes rapid and cost-effective insights. In this study, we conduct an extensive examination of the multiplexing capabilities of near-infrared (NIR) FLI within a scattering medium that emulates biological tissues. We introduce an efficient, novel Monte Carlo (MC) simulation approach that describe the scattering behavior of fluorescent photons within turbid media. Subsequent application of phasor analyses enabled multiplexing of distinct targets within a single image. Leveraging the cutting-edge single-photon avalanche diode (SPAD) time-gated camera, SPAD512S, to perform experimental wide-field FLI in the NIR regime, we present the multiplexing of dual targets within a single image to a depth of 1 cm within tissue-like phantoms.
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Author(s): Konstantine Cheishvili, Technische Univ. Delft (Netherlands)
9 April 2024 • 18:10 - 20:00 CEST | Galerie Schweitezer, Niveau/Level 0
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We have developed an extensive wavelength range DLS-OCT system designed for measuring the concentration and wavenumber-dependent diffusive dynamics of particle suspensions in a single acquisition and fixed geometry. We successfully utilized our system to measure the wavenumber-dependent collective diffusion coefficient in a concentrated silica suspension and to estimate particle size. We demonstrate the feasibility of measuring both the collective and self-diffusion terms, even in monodisperse particle suspensions, by leveraging optical polydispersity. Additionally, we have introduced a novel method for determining particle size polydispersity in concentrated suspensions using the ratio of self and collective mode amplitudes. Our technique exhibits significantly greater sensitivity to smaller particles compared to the standard cumulant analysis typically used in dilute suspensions. Furthermore, when both collective and self-diffusion are measured, it becomes possible to obtain accurate estimates of particle size and volume fraction at a single wavenumber, enabling depth-resolved measurements.
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Author(s): Rowan W. Sanderson, Kai L. Metzner, The Univ. of Western Australia (Australia); Brendan F. Kennedy, The Univ. of Western Australia (Australia), Nicolaus Copernicus Univ. (Poland)
9 April 2024 • 18:10 - 20:00 CEST | Galerie Schweitezer, Niveau/Level 0
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Optical elastography techniques utilise light to map the mechanical properties of tissue by measuring tissue deformation in response to an applied load. As is the case for most medical imaging techniques, the ongoing development of optical elastography is dependent on the parallel development of suitable tissue-mimicking phantoms. As optical elastography is still an emerging modality, existing phantoms are rudimentary and fail to capture the complex mechanical and structural heterogeneity of tissue. In this work, we present a methodology for the design of detailed tissue-mimicking phantoms that accurately represent the structures, surface profiles, and mechanical contrast of breast tissue specimens.
13006-74
Author(s): Farzin Ghane Golmohamadi, Franz-Josef Schmitt, Amna Mehmood, Hoang Phan, Patrick Galert, Jan Laufer, Martin-Luther-Univ. Halle-Wittenberg (Germany)
9 April 2024 • 18:10 - 20:00 CEST | Galerie Schweitezer, Niveau/Level 0
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Genetically expressed fluorescent proteins are attractive contrast agents for molecular photoacoustic tomography. Pump-probe excitation, relying on the excitation of fluorescent proteins with a pump pulse and the subsequent stimulation of fluorescent emissions using a time-delayed probe pulse, is a simple experimental method that has been shown to provide fluorophore specific contrast. We developed a theoretical model to simulate photoacoustic pump probe signal generation in fluorophores. The results were validated experimentally in solutions of red fluorescent proteins and dyes using a photoacoustic spectrometer. The modeling results show good agreement with experimental data when scanning the pump wavelength across the absorption spectrum of fluorophores and when the pump probe time delay is varied. The results suggest that fluorescent proteins can be used as biosensors in PA imaging to measure biophysical parameters within tissue such as pH.
13006-75
Author(s): Ryan Muddiman, National Univ. of Ireland, Maynooth (Ireland)
9 April 2024 • 18:10 - 20:00 CEST | Galerie Schweitezer, Niveau/Level 0
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The capability of Raman spectroscopy for biological cell classification has been previously reported and is shown to be well suited for research purposes. The implementation in the clinical setting for such tasks as cell counting and pathology is prohibited by the required acquisition time due to the low scattering cross section present. In this work, we present a study on the capability of broadband coherent anti-Stokes Raman scattering (BCARS) using a fiber laser, for cell classification. The improvements in acquisition time afforded by the coherent process in BCARS could potentially allow for clinical implementation but there are known drawbacks in BCARS such as the quadratic concentration dependence and nonresonant background. We provide a comparison of the resulting classification with spontaneous Raman and also provide information on the mitigation of the drawbacks in BCARS.
13006-76
Author(s): MohammadSadegh Vafaeinezhad, Carl Messerschmidt, Matteo Calvarese, Parijat Barman, Abbe Ctr. of Photonics, Friedrich-Schiller-Univ. Jena (Germany), Leibniz-Institut für Photonische Technologien e.V. (Germany); Denis Akimov, Leibniz-Institut für Photonische Technologien e.V. (Germany); Tobias Meyer-Zedler, Abbe Ctr. of Photonics, Friedrich-Schiller-Univ. Jena (Germany), Leibniz-Institut für Photonische Technologien e.V. (Germany); Anna Mühlig, Orlando Guntinas-Lichius, Universitätsklinikum Jena (Germany); Michael Schmitt, Abbe Ctr. of Photonics, Friedrich-Schiller-Univ. Jena (Germany); Juergen Popp, Abbe Ctr. of Photonics, Friedrich-Schiller-Univ. Jena (Germany), Leibniz-Institut für Photonische Technologien e.V. (Germany)
9 April 2024 • 18:10 - 20:00 CEST | Galerie Schweitezer, Niveau/Level 0
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In this work, we present a BCARS spectral imaging system and its application to biomedical research. This setup combines a chirped pulse amplification laser with the generation of supercontinuum white light in bulk crystals and fibers to produce a broad-spectrum high power and low noise excitation source. The efficient and stable white light generation in All Normal Dispersion range of Photonics Crystal Fiber (PCF) has been simulated and experimentally demonstrated. Biomedical imaging applications utilizing a combination of line scanning mosaic imaging and spectrograph detection and data analysis approaches are presented.
13006-77
Author(s): Pedro Braga-Fernandes, Vera Cardoso, INL - International Iberian Nanotechnology Lab. (Portugal), Univ. de Aveiro (Portugal); Christian Maibohm, Jana B. Nieder, INL - International Iberian Nanotechnology Lab. (Portugal); Johannes W. Goessling, Univ. de Aveiro (Portugal); Martin Lopez-Garcia, INL - International Iberian Nanotechnology Lab. (Portugal)
9 April 2024 • 18:10 - 20:00 CEST | Galerie Schweitezer, Niveau/Level 0
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The high and unique demand for materials and their processing in nanophotonic technologies negatively impacts the environment. In this context, using biological systems as sources of photonic nanomaterial arose as an efficient alternative for light manipulation at the nanoscale, providing high-quality and sustainable materials for nanotechnological applications. Within the vast family of biomaterials suitable for photonic application, diatoms are one of the most attractive options due to their nanostructured biosilica exoskeleton – the frustule. Diatoms are a group of unicellular photosynthetic microalgae living in most aquatic and humid habitats. They naturally produce a species-dependant nano-porous pattern in their silicate-based frustule, which is easy to isolate and, therefore, easy to use as scaffolds for nanophotonic applications. In this paper, we introduce diatoms as a source of high-quality slab photonic crystals with incorporated micro-photoluminescence properties, which opens the doors for high-quality bio-produced emission devices.
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Author(s): Subham Adak, Abbe Ctr. of Photonics, Friedrich-Schiller-Univ. Jena (Germany), Leibniz-Institut für Photonische Technologien e.V. (Germany); Anooj Thayyil Raveendran, Leibniz-Institut für Photonische Technologien e.V. (Germany); Jürgen Popp, Abbe Ctr. of Photonics, Friedrich-Schiller-Univ. Jena (Germany), Leibniz-Institut für Photonische Technologien e.V. (Germany); Christoph Krafft, Leibniz-Institut für Photonische Technologien e.V. (Germany)
9 April 2024 • 18:10 - 20:00 CEST | Galerie Schweitezer, Niveau/Level 0
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We describe a new technique called widefield optical photothermal infrared (OPTIR) microscopy that selectively images chemical bonds in living biological and material samples. Our widefield OPTIR microscope captures images of a large specimen area in milliseconds at submicron spatial resolution. We use a pulsed visible (LED) light for high-throughput, widefield sensing of the transient photothermal effect induced by absorption of single mid-IR pulses of a pump laser. Our system can image a field of approximately 300×300 μm^2 at 200 ms frame rate using a 5 mega-pixel CMOS camera. In our presentation, we will demonstrate the capabilities of our widefield OPTIR microscope by characterizing and imaging biological specimens and nanometer-scale polymer films. Furthermore, we will also demonstrate our latest development of our widefield OPTIR microscope for rapid multi-spectral imaging of biological samples.
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Author(s): Rui Yuan, Robert A. Byers, Dmitry G. Revin, Stephen J. Matcher, The Univ. of Sheffield (United Kingdom)
9 April 2024 • 18:10 - 20:00 CEST | Galerie Schweitezer, Niveau/Level 0
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Atopic dermatitis (AD), characterized by itchiness and inflammation, often results in increased skin thickness. Traditional treatments with topical corticosteroids may compound this effect. Addressing the need for accurate epidermal measurement and the slow acquisition times of previous methods, we developed a high-speed OCT system utilizing a 1.67 MHz FDML laser and a MEMS scanner, enabling 0.1-second scans that simplify patient compliance. The system has an axial resolution of 13.2 µm and a lateral resolution of 44.2 µm in air and a maximum field of view of 2.9 mm x 2.9 mm. An automatic segmentation algorithm was developed to delineate the skin surface and epidermal-dermal junction. Initial findings show the epidermal layer ranges from 120 to 130 micrometers on dorsal hand skin, with a detailed epidermal thickness map for visualization of skin structure. A more efficient and patient-friendly approach is offered for evaluating AD severity and treatment response.
13006-80
Author(s): Pooja Anantha, Johns Hopkins Univ. (United States); Annalisa Convertino, Institute for Microelectronics and Microsystems, National Research Council of Italy (Italy); Emanuela Saracino, Institute for Organic Synthesis and Photoreactivity, National Research Council of Italy (Italy); Ishan Barman, Johns Hopkins Univ. (United States)
9 April 2024 • 18:10 - 20:00 CEST | Galerie Schweitezer, Niveau/Level 0
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Astrocytes, integral glial cells within the CNS, have garnered increased attention for their pivotal roles in brain and spinal cord function, including information processing, neurotransmitter balance, and synaptic modulation. Their dysregulation has been implicated in various neurological disorders. Despite their acknowledged significance, our comprehension of astrocytes remains incomplete, particularly regarding their intricate morphology. Quantitative phase imaging (QPI) offers a novel approach, enabling label-free imaging of biological samples. In our study, we investigated the impact of silicon nanowire (SiNW) substrates on rat cortical astrocyte morphology, aiming to understand how this novel substrate influences astrocyte morphology compared to traditional glass substrates. The novelty lies in utilizing QPI to image astrocytes on nanostructured substrates such as SiNW substrates. Astrocytes cultured on SiNW substrates displayed a “star-like” morphology typically found in vivo. This leads to several opportunities for future studies such as quantification of morphological features of astrocytes on SiNW substrates.
13006-81
Author(s): Ilya Balmages, Riga Technical Univ. (Latvia); Aigars Reinis, Pauls Stradinš Clinical Univ. Hospital (Latvia), Riga Stradinš Univ. (Latvia); Svjatoslavs Kistkins, Pauls Stradinš Clinical Univ. Hospital (Latvia); Janis Liepins, Maksym Pogorielov, Viktoriia Korniienko, Kateryna Diedkova, Univ. of Latvia (Latvia); Dmitrijs Bliznuks, Riga Technical Univ. (Latvia); Alexey Lihachev, Ilze Lihacova, Univ. of Latvia (Latvia)
9 April 2024 • 18:10 - 20:00 CEST | Galerie Schweitezer, Niveau/Level 0
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Our previous studies have shown that laser speckle imaging with sensitive subpixel correlation analysis is able to detect microbial growth activity and the pattern of colony growth. In the current study, we demonstrate the potential of this method to analyze fungal growth. We compare the characteristics of the signals obtained from bacteria and fungi. The obtained results will help to improve the parameters of the speckle image acquisition system and the signal processing algorithms useful for microorganism (both eukaryotic and prokaryotic) growth analyses and speeding up and facilitating microbiological diagnostics. Acknowledgments This research is funded by the Latvian Council of Science funded project “Dynamic laser speckle imaging for evaluation of fungal growth activity” (agreement No. lzp-2022/1-0247).
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Author(s): Irma Dumbryte, Donatas Narbutis, Vilnius Univ. (Lithuania); Maria Androulidaki, Institute of Electronic Structure and Laser, Foundation for Research and Technology-Hellas (Greece); Elena Jasiuniene, Kaunas Univ. of Technology (Lithuania); Arturas Vailionis, Stanford Univ. (United States); Saulius Juodkazis, Swinburne Univ. of Technology (Australia); Mangirdas Malinauskas, Vilnius Univ. (Lithuania)
9 April 2024 • 18:10 - 20:00 CEST | Galerie Schweitezer, Niveau/Level 0
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The study aimed to combine an X-ray micro-computed tomography (µCT), enhanced with convolutional neural network (CNN) assisted voxel classification and volume segmentation, with photoluminescence (PL) and micro-Raman spectroscopy (µ-RS) for tooth structural integrity assessment at the microcrack (MC) site, and verify this approach with extracted human teeth. The samples were first examined using an X-ray µCT and segmented with CNN to identify enamel, dentin, and cracks. Secondly, buccal and palatal teeth surfaces with MCs and sound areas were selected to obtain fluorescence spectra illuminated with laser exposure wavelengths: 325nm (CW) and 266nm (0.5 ns pulsed). Thirdly, chemical composition inside the crack and the difference from the good area were determined using µ-RS. The proposed approach revealed differences in the material along the crack line, with a variation in the hydroxyapatite crystals at the cracked versus sound area. This suggests a possible loss of tooth structural integrity at the MC site.
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Author(s): Damian Mendroch, Niklas Bauer, David Harings, Technische Hochschule Köln (Germany); Alexander Heisterkamp, Leibniz Univ. Hannover (Germany)
9 April 2024 • 18:10 - 20:00 CEST | Galerie Schweitezer, Niveau/Level 0
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This work showcases an innovative approach to enhancing images acquired by a linear optical coherence tomography system. Multiple types of filtering combat image noise, while a sophisticated deconvolution algorithm based on maximum likelihood estimation compensates for degradation due to system blur. The results yield reduced noise, improved resolution and better structure visibility. Multiple examples of technical and biological samples demonstrate the significant increase in image quality.
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Author(s): Niklas Bauer, Damian Mendroch, Jan Matrisch, David Harings, Technische Hochschule Köln (Germany); Alexander Heisterkamp, Leibniz Univ. Hannover (Germany)
9 April 2024 • 18:10 - 20:00 CEST | Galerie Schweitezer, Niveau/Level 0
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This publication introduces a prototype of a fiber-based linear optical coherence tomography system (LOCT) that can be used for economical retinal screening in ophthalmology. The system uses standard off-the-shelf components to reduce production costs, complexity, and adjustment efforts while providing high-quality imaging of retinal structures. We present the results of A- and B-scans of technical samples and an artificial eye model that was conducted to assess the system's performance regarding axial resolution, imaging depth, and dispersion compensation. The study's findings suggest that LOCT is a cost-effective solution for ophthalmology and shows great potential for monitoring the progression of retina-related diseases such as Glaucoma or age-related macular degeneration.
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Author(s): Artjoms Suponenkovs, Dmitrijs Bliznuks, Riga Technical Univ. (Latvia); Ilze Lihacova, Univ. of Latvia (Latvia)
9 April 2024 • 18:10 - 20:00 CEST | Galerie Schweitezer, Niveau/Level 0
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The importance of bacteriological analysis is growing. The standard approach - disk-diffusion test allows estimating the susceptibility of bacteria to an antibiotic. It is possible to assess the susceptibility of bacteria by using the inhibition zone diameter. The bacteriologist usually measures the inhibition zone diameter manually. The proposed methods aim to perform automatic inhibition zone measurement that saves bacteriologists time and lowers patient treatment-related risks. The presented noncontact approach is based on computer vision technologies and addresses the challenges of image pre-processing, antibiotic disc localization, inhibition zones segmentation and measurement. The experimental part contains the results and comparisons of inhibition zones measurement methods. The experimental results show the advantages and disadvantages of existing methods. The existing approaches face the challenges of low contrast, overlapping zones, weak edges of a zone, non-circular inhibition shape and double inhibition zones. This work provides solutions to these problems.
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Author(s): Aytac Ozkan, Technische Univ. Berlin (Germany); Violeta Madjarova, Elena V. Stoykova, Institute of Optical Materials and Technologies (Bulgaria); Thomas Sikora, Technische Univ. Berlin (Germany)
9 April 2024 • 18:10 - 20:00 CEST | Galerie Schweitezer, Niveau/Level 0
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We introduce a novel enhancement method for Optical Coherence Tomography (OCT) images, combining a "shifted steered mixture of experts" with an autoencoder for simultaneous denoising and super-resolution. This method uniquely avoids retraining for different scaling factors and allows on-the-fly adjustments for edge sharpening or defocusing. Tested on our in-house OCT dataset, it provides superior denoising and edge preservation without the computational burden of traditional deep learning approaches. Performance assessments demonstrate its advantage over current state-of-the-art methods in both subjective and objective (PSNR, perceptual metrics) evaluations.
13006-87
Author(s): Van Phuc Nguyen, Univ. of Michigan Kellogg Eye Ctr. (United States); Xueding Wang, Univ. of Michigan (United States); Yannis M. Paulus, Univ. of Michigan Kellogg Eye Ctr. (United States)
9 April 2024 • 18:10 - 20:00 CEST | Galerie Schweitezer, Niveau/Level 0
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Choroidal neovascularization (CNV) stands as the leading cause of vision loss in individuals afflicted with macular degeneration. Detecting CNV early and precisely determining its location accurately remains a formidable challenge with existing imaging methods. In this research, we introduce a multimodal triple imaging approach incorporating photoacoustic microscopy (PAM), optical coherence tomography (OCT), and fluorescence microscopy (FM) powered by gold nanoparticles to improve the precision of CNV diagnosis by harnessing the strengths of each modality. Nevertheless, conventional gold nanoparticle contrast agents have long-term safety concerns due to their accumulation in the liver and spleen. To overcome this issue, we have devised an innovative contrast agent founded on ultra-small gold nanospheres (GNS), which not only improves renal excretion but also enhances the contrast in PAM, OCT, and FM imaging. These ultra-small GNS were fabricated using pulsed laser ablation methods and subsequently surface-modified with CALNN peptides and cysteamine molecules, forming gold nanochains (GNC). Further surface conjugation with RGD peptides and indocyanine green (ICG) fluorescent dye
13006-88
Author(s): Julian Plitzko, Friedrich-Schiller-Univ. Jena (Germany)
9 April 2024 • 18:10 - 20:00 CEST | Galerie Schweitezer, Niveau/Level 0
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To investigate functionalized polymer-based nanoparticles (NPs) within cells and tissue, molecular selective microspectroscopic techniques are well suited. In our research we utilize confocal fluorescence microscopy and coherent anti-Stokes Raman scattering (CARS) microscopy to study the localization of NPs in cells and tissue. Raman spectroscopy in combination with two-dimensional correlation analysis (2DCOS) is utilized for the characterization of polymers, NPs, and drugs prior to its analysis in biological environments. Additionally, we utilize fluorescence lifetime microscopy (FLIM) to investigate the release of the encapsulated drug compounds of the functionalized NPs.
13006-89
Author(s): David Harings, Niklas Bauer, Damian Mendroch, Technische Hochschule Köln (Germany); Holger Lubatschowski, Leibniz Univ. Hannover (Germany); Uwe Oberheide, Technische Hochschule Köln (Germany)
9 April 2024 • 18:10 - 20:00 CEST | Galerie Schweitezer, Niveau/Level 0
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This approach offers continuous near-infrared fundus imaging while eliminating pupil contraction and minimizing reflections through polarized illumination. Utilizing a controllable screen to generate individual fixation stimuli allows for dynamic eye movements during imaging. This method lends itself to creating advanced possibilities within optical coherence tomography (OCT). In addition, the system might detect visual field defects by identifying missing movements in the fundus image during recording.
13006-90
Author(s): Mihai-Stefan M. Duma, Ralph-Alexandru Erdelyi, Univ. Politehnica Timisoara (Romania); Anne-Marie Heredea, Rodica Heredea, Univ. de Medicina si Farmacie "Victor Babes" din Timisoara (Romania); Virgil-Florin Duma, Univ. "Aurel Vlaicu" din Arad (Romania), Univ. Politehnica Timisoara (Romania); Cosmin G. Sinescu, Univ. de Medicina si Farmacie "Victor Babes" din Timisoara (Romania)
9 April 2024 • 18:10 - 20:00 CEST | Galerie Schweitezer, Niveau/Level 0
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The present study explores the capabilities that OCT has to serve for the study of biofilm deposition, eventually in conjunction with other investigation methods. The investigations were carried out on two directions: (i) to evaluate different methods for manufacturing dental prostheses, with the scope to identify a correlation between the design of the fixed partial prostheses pontic’s and the cytodiagnostic of the covering gingiva of the edentulous ridge and the gingival tissue around the abutments of the fix partial prostheses; (ii) to analyze the efficiency of different cleaning methods of dentistry equipment by assessing biofilm deposition in different types of tubes employed in such equipment (considering the inside of tubes of different diameters chosen for their long usage period in order to generate significant imaging results). In both studies an in-house developed Swept Source (SS) OCT system centered at 1300 nm, with an axial resolution of 15 µm was utilized. The results showed that OCT was capable to offer quantitative evaluations of the width of biofilm deposition, complementing for example (conventional) microbiology investigations.
13006-91
Author(s): Kirill Buiankin, Edwin van der Pol, Ton G. van Leeuwen, Amsterdam UMC (Netherlands)
9 April 2024 • 18:10 - 20:00 CEST | Galerie Schweitezer, Niveau/Level 0
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This research focuses on enhancing the sensitivity of flow cytometry for the detection of extracellular vesicles (EVs), particularly those smaller than 100-200 nm. Extracellular vesicles play a crucial role in cellular waste disposal and intercellular communication, serving as disease biomarkers. Conventional flow cytometers often lack the required sensitivity for accurately detecting such small EVs. The study proposes the integration of Optical Coherence Tomography (OCT) with flow cytometry to improve sensitivity and reduce background signals. We first demonstrate the proof of concept with a commercial OCT system and conduct a comparative analysis alongside established commercial flow cytometers using a solution of polystyrene beads with known sizes. We determined the lower limit of detection (LOD) for the current OCT system to be a polystyrene bead of 600 nm diameter. Using this LOD, we describe potential hardware and software optical and fluidic modifications and calculate the potential LOD of an adapted OCT system to be the detection of an EV of 100 nm in diameter.
13006-92
Author(s): Hyeonsoo Bae, Leibniz-Institut für Photonische Technologien e.V. (Germany), Abbe Ctr. of Photonics, Friedrich-Schiller-Univ. Jena (Germany); Matteo Calvarese, Tobias Meyer-Zedler, Abbe Ctr. of Photonics, Friedrich-Schiller-Univ. Jena (Germany), Leibniz-Institut für Photonische Technologien e.V. (Germany); Anna Muehlig, Universitätsklinikum Jena (Germany); Chenting Lai, Karl Reichwald, Bernhard Messerschmidt, Grintech GmbH (Germany); Orlando Guntinas-Lichius, Universitätsklinikum Jena (Germany); Michael Schmitt, Abbe Ctr. of Photonics, Friedrich-Schiller-Univ. Jena (Germany); Juergen Popp, Abbe Ctr. of Photonics, Friedrich-Schiller-Univ. Jena (Germany), Leibniz-Institut für Photonische Technologien e.V. (Germany)
9 April 2024 • 18:10 - 20:00 CEST | Galerie Schweitezer, Niveau/Level 0
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Since its inception in the 1980s, CARS microscopy has greatly advanced, offering label-free imaging of molecular distributions and incorporating ultra-short pulse lasers to facilitate modalities like SHG and TPEF. Translating these nonlinear imaging methods for endoscopic clinical diagnostics involves challenges such as reducing background noise, miniaturizing probes, optimizing light transmission, and advancing data processing. Addressing these, we introduce two nonlinear endoscopic systems: a multicore fiber-based probe with proximal remote scanning and a custom reconstruction algorithm that enhances image readability, and a double-core double clad fiber based endoscope that allows background-free laser delivery and sub cellular resolution imaging. These systems promise potential to meet clinical requirements by providing detailed imaging performance comparable to conventional nonlinear microscopy.
13006-93
Author(s): Richard Grohs, Anja Silge, Leibniz-Institut für Photonische Technologien e.V. (Germany), Forschungcampus InfectoGnostics (Germany); Uwe Glaser, Leibniz-Institut für Photonische Technologien e.V. (Germany); Karina Weber, Leibniz-Institut für Photonische Technologien e.V. (Germany), Forschungcampus InfectoGnostics (Germany); Oleg Ryabchykov, Leibniz-Institut für Photonische Technologien e.V. (Germany), Forschungcampus InfectoGnostics (Germany), Biophotonics Diagnostics GmbH (Germany); Franziska Hornung, Stefanie Deinhardt-Emmer, Bettina Löffler, Universitätsklinikum Jena (Germany); Jürgen Popp, Leibniz-Institut für Photonische Technologien e.V. (Germany), Forschungcampus InfectoGnostics (Germany), Abbe Ctr. of Photonics, Friedrich-Schiller-Univ. Jena (Germany)
9 April 2024 • 18:10 - 20:00 CEST | Galerie Schweitezer, Niveau/Level 0
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Diagnostic Raman spectroscopy offers a rapid approach for pathogen detection and phenotypic antimicrobial susceptibility testing. Following isolation, two common bloodstream infection bacteria, Escherichia coli and Staphylococcus aureus, were treated with appropriate antibiotic concentrations and analyzed using On-Chip Raman spectroscopy. Using photonic data analysis, the spectral signals are translated into antibiograms.
13006-94
Author(s): Stella Greiner, Abbe Ctr. of Photonics, Friedrich-Schiller-Univ. Jena (Germany); Tobias Meyer-Zedler, Marko Rodewald, Abbe Ctr. of Photonics, Friedrich-Schiller-Univ. Jena (Germany), Leibniz-Institut für Photonische Technologien e.V. (Germany); Michael Schmitt, Abbe Ctr. of Photonics, Friedrich-Schiller-Univ. Jena (Germany); Ute Neugebauer, Abbe Ctr. of Photonics, Friedrich-Schiller-Univ. Jena (Germany), Leibniz-Institut für Photonische Technologien e.V. (Germany); Orlando Guntinas-Lichius, Universitätsklinikum Jena (Germany); Franziska Hornung, Stefanie Deinhardt-Emmer, Friedrich-Schiller-Univ. Jena (Germany); Juergen Popp, Abbe Ctr. of Photonics, Friedrich-Schiller-Univ. Jena (Germany), Leibniz-Institut für Photonische Technologien e.V. (Germany); Bettina Loeffler, Institut für Medizinische Mikrobiologie, Universitätsklinikum Jena (Germany)
9 April 2024 • 18:10 - 20:00 CEST | Galerie Schweitezer, Niveau/Level 0
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Two-photon excited fluorescence lifetime imaging microscopy (FLIM) is a powerful tool for visualizing minute changes in the chemical makeup of biological samples in a spatially resolved manner. Here, we present three applications of this concept from the field of biomedicine: (1) detection of tumor tissue in head and neck cancer samples, (2) sepsis characterization in mouse kidney tissue, and (3) characterization of the host response to influenza infection in lung tissue.
13006-95
Author(s): Denis F. Leroux, Régis Montvernay, Corinne Davenas, Yann LeBihan, Corine Fulchiron, bioMérieux SA (France)
9 April 2024 • 18:10 - 20:00 CEST | Galerie Schweitezer, Niveau/Level 0
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Identification of after-culture microbial species using a low-cost fully automated system would strongly benefit Low Income Countries, as traditional culture plate reading for the purpose of species identification currently requires train professionals and remains mostly manual, while other automated identification methods are costly. Our application is the label-free identification of uropathogens from bacterial colonies images directly on a non-chromogenic culture medium collection. With a frugal innovation mindset, we are developing a simple diagnostic system based on a filter wheel and a smartphone-driven CMOS camera. We are reporting for the first-time performance of identification for true clinical samples and compare it to samples issued from a strain collection. Also, the capability to classify the five most-prevalent uropathogens (MPU) in presence of ten low-prevalent species (LPU) is evaluated. Two machine learning classification approaches are compared: classical or probabilistic SVM (Platt method) using solely a 1-D vector of intensities without any morphological predictors at this stage. Shortcomings of the logistic regression approach are highlighted.
13006-96
Author(s): Lázaro P. Medeiros Neto, DermoPROBES (Brazil), Fundação de Amparo à Pesquisa do Estado de São Paulo (Brazil)
9 April 2024 • 18:10 - 20:00 CEST | Galerie Schweitezer, Niveau/Level 0
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The evaluation of endogenous hyaluronic acid (HA) in the skin is important to understand the ageing process and analyze the effectiveness of dermocosmetics. Thus, this study evaluated the skin of 10 individuals between 51 and 65 years old, before and after the application of a dermocosmetic to stimulate HA, using the confocal Raman spectroscopy technique (Gen2 - Rivers Diagnostic). Six marker peaks for the presence of HA were observed: 1076, 1084, 1096, 1126, 1132 and 1208 cm-1, which allowed evidence of increases in their intensity after 30 and 60 days of use of the product, indicating an increase in the concentration of HA. Therefore, the in vivo assessment of cutaneous HA can facilitate the understanding of the role of dermocosmetics in skin aging, allowing for more effective treatments.
13006-97
Author(s): Mónica Canabal-Carbia, Irene Estévez, Ivan Montes-González, Juan Campos, Angel Lizana, Univ. Autònoma de Barcelona (Spain)
9 April 2024 • 18:10 - 20:00 CEST | Galerie Schweitezer, Niveau/Level 0
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We propose an analysis of the polarimetric attributes of biological tissue (dichroism, retardance and polarization). This analysis aims to characterize the intrinsic properties of the tissue and its polarimetric response when interacting with light. These polarimetric parameters are obtained through the experimental measurement of the Mueller matrix of the sample. We study the suitability of various sets of metrics for identification of biological tissues, by means of different pseudo-coloration techniques, highlighting some metrics such as depolarization. The results shown in an ex-vivo cow brain sample suggest the convenience of certain parameters which may be of interest in multiple biomedical applications.
13006-98
Author(s): Muhammad Zubair, King Abdullah Univ. of Science and Technology (Saudi Arabia); Aqeel Ur-Rehman, Information Technology Univ. of the Punjab (Pakistan)
9 April 2024 • 18:10 - 20:00 CEST | Galerie Schweitezer, Niveau/Level 0
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Laser speckle contrast imaging (LSCI) is extensively used imaging technique in bio-medical engineering with optimization of laser sources and camera configurations. With an emphasis on type 2 diabetes, which affects 90% of patients globally, LSCI is useful in diagnosis of illness. Applications of LSCI, such as microvascular monitoring and wound healing assessment are examined in diabetes research. LSCI combines hardware design with useful healthcare applications, offering the opportunity for early diagnosis and tailored therapy in diabetes management. Researchers and medical practitioners looking to use LSCI technology to solve diabetes-related issues will find this evaluation to be a useful resource.
13006-99
Author(s): Jawad Talekkara Pandayil, Politecnico di Torino (Italy), Fondazione Links (Italy); Nadia G. Boetti, Fondazione Links (Italy); Lorenzo Cortese, ICFO - Institut de Ciències Fotòniques (Spain); Davide Janner, Politecnico di Torino (Italy); Turgut Durduran, ICFO - Institut de Ciències Fotòniques (Spain), ICREA - Institució Catalana de Recerca i Estudis Avançats (Spain)
9 April 2024 • 18:10 - 20:00 CEST | Galerie Schweitezer, Niveau/Level 0
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Development of optical quality bioresorbable fibers is an emerging area of study where researchers are trying to advance the field by assessing the suitability of these fibers for various biomedical applications. These types of fiber implants dissolve in the human body over a clinically relevant time scale eliminating the need for extraction surgeries. We conducted both ex-vivo and in vivo diffuse correlation spectroscopic studies using our fibers to measure blood flow and a preliminary trial to integrate a biocompatible electrode material on the fiber for electrical signal measurements. The results demonstrated the potential of Calcium Phosphate glass-based fiber-optic devices in future physiological monitoring applications which can be implanted inside the body without the need of an explant procedure. Acknowledgement: This project has received funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Sklodowska-Curie grant agreement No 860185.
13006-100
Author(s): Blaž Cugmas, Univ. of Latvia (Latvia), Vetamplify SIA (Latvia); Eva Štruc, Vetamplify SIA (Latvia); Inese Berzina, Mindaugas Tamosiunas, Univ. of Latvia (Latvia); Thierry Olivry, Nextmune AB (Sweden); Miran Bürmen, Peter Naglic, Univ. of Ljubljana (Slovenia)
9 April 2024 • 18:10 - 20:00 CEST | Galerie Schweitezer, Niveau/Level 0
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Pollen monitoring is helpful for medical purposes, weather or crop forecasting, and climate change analysis. Pollens are also important aeroallergens in veterinary medicine, causing atopic dermatitis in dogs and horses, and skin disease, seasonal rhinitis, and asthma in cats. In this study, we develop a lensless microscope and investigate its prospects for real-time detection and identification of six pollens relevant to veterinary medicine. By a systematic selection of a light source and imaging sensor, we present an optimal design for capturing digital holograms. Various light sources are studied based on parameters, including the source-object distance, temporal and spatial coherence, wavelength, and optical power for pulsed illumination. We propose an automated pollen detection and identification procedure based on a deep learning model for hologram classification. The proposed procedure is validated by an experienced pathologist using a conventional commercially available light microscope.
13006-101
Author(s): Kiran Philip Isaac, Sujatha N. Unni, Indian Institute of Technology Madras (India)
9 April 2024 • 18:10 - 20:00 CEST | Galerie Schweitezer, Niveau/Level 0
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Ampicillin is used in this study to test the susceptibility of Escherichia coli (E. coli) strain ATCC25922 to it. The autofluorescence characteristics of the bacteria are used at excitation wavelengths of 360 nm and emission wavelengths of 450 nm. The antibiotic sensitivity was measured by comparing the fluorescence intensities of antibiotic-exposed samples to a control (E. coli in PBS without antibiotics). Standard procedures such as disc diffusion and broth microdilution are used to confirm the findings.
13006-102
Author(s): Bryan M. Hennelly, Keela Kessie, Ryan Muddiman, Rachel Dillon, Kevin Kavanagh, Trinidad Velasco-Torrijos, National Univ. of Ireland, Maynooth (Ireland)
9 April 2024 • 18:10 - 20:00 CEST | Galerie Schweitezer, Niveau/Level 0
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Resistant microorganisms do not respond to conventional treatments causing lethal infections, especially in immunocompromised people. The urgent need for fast, reliable, and highly specific diagnostic methods to control this silent pandemic is evident. Broadband Coherent anti-Stokes Raman Spectroscopy (bCARS) is a powerful, highly sensitive spectroscopic technique that can be used for obtaining detailed molecular spectra and imaging of cells and tissues. We have prepared pathogen specific glycoprobes and are currently optimizing the detection, chemical imaging, and classification of fungal pathogens such as Candida albicans with a bCARS setup.
13006-103
Author(s): Bryan M. Hennelly, Timothy J. McNamara, Ryan Muddiman, National Univ. of Ireland, Maynooth (Ireland)
9 April 2024 • 18:10 - 20:00 CEST | Galerie Schweitezer, Niveau/Level 0
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Hyperspectral imaging based on vibrational spectroscopy offers detailed, label-free chemical insights. Techniques like Near-Infrared (NIR), Fourier-Transform Infrared (FTIR), and Raman spectroscopy have bandwidth and speed constraints, particularly with water-based samples. Coherent Raman imaging, especially Stimulated Raman used with Second Harmonic Generation has recently been applied to produce virtual histology images without staining as well as segmenting cell features via spectral phasor analysis. Here we explore Broadband Coherent anti-Stokes Raman scattering (BCARS) as a method to produce high-quality virtually stained false-colour images of biological cells. BCARS enables fast, high-resolution (<10cm-1) and broad bandwidth (400-4000cm-1) bio-imaging but faces issues like low signal-to-noise ratio. This paper advances previous BCARS work, exploring new false-color image generation methods for diverse biological samples.
13006-104
Author(s): Minnu Varghese, Technische Univ. München (Germany)
9 April 2024 • 18:10 - 20:00 CEST | Galerie Schweitezer, Niveau/Level 0
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Optical Coherence Tomography (OCT) has become a ground-breaking method in medical imaging. This study covers all aspects of OCT, including its concepts, uses, and the considerable influence it has had on healthcare. OCT provides high-resolution, non-invasive imaging, making it a useful tool in ophthalmology, cardiology, dermatology, and other medical specialities. The underlying concepts underpinning OCT, such as its functioning principles, imaging modalities, and the most recent breakthroughs in the area were all looked at thoroughly. The study also goes through OCT's clinical uses, emphasizing its significance in the early identification, diagnosis, and monitoring of a variety of medical disorders. Furthermore, the potential future paths and advances in OCT technology that have the potential to improve diagnostic capabilities and widen clinical utility are also investigated. This thorough review seeks to offer a clearer knowledge of OCT's importance in modern medicine and to stimulate more study and development in this interesting topic.
13006-112
Author(s): Thanh Dat Le, Chonnam National Univ. (Vietnam); Mansik Jeon, Kyungpook National Univ. (Korea, Republic of); Changho Lee, Chonnam National Univ. (Korea, Republic of)
9 April 2024 • 18:10 - 20:00 CEST | Galerie Schweitezer, Niveau/Level 0
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Optoacoustic microscopy (OAM) is emerging as a promising biomedical imaging modality that integrates the advantages of optical and ultrasound imaging to visualize biological tissues with high sensitivity and spatial resolution. Our study demonstrated OAM with 3D Hessian filter segmentation to quantify microvasculatures within tumors in mice over 11 days. Analyzing diverse quantitative parameters such as vessel diameter, density, perfusion, and complexity yielded crucial insights into tumor growth dynamics and angiogenesis. Our result showed OAM and 3D Hessian filter segmentation's potential for rigorously quantifying tumor progression and guiding the development of precise cancer therapies.
Session 7: Optical Coherence Tomography I
10 April 2024 • 08:30 - 10:20 CEST | Etoile B, Niveau/Level 1
Session Chair: Shau Poh Chong, National Univ. of Singapore (Singapore)
13006-33
Author(s): Rowan W. Sanderson, Ken Y. Foo, Renate Zilkens, Imogen Boman, The Univ. of Western Australia (Australia), Harry Perkins Institute of Medical Research (Australia); Lee Jackson, Jose Cid Fernandez, Fiona Stanley Hospital (Australia); Benjamin F. Dessauvagie, PathWest Lab. Medicine WA, Fiona Stanley Hospital (Australia), The Univ. of Western Australia (Australia), Clinipath Pathology (Australia); Chris Yeomans, Anmol Rijhumal, PathWest Lab. Medicine WA, Fiona Stanley Hospital (Australia); Saud Hamza, Fiona Stanley Hospital (Australia); Christobel M. Saunders, The Univ. of Melbourne (Australia), The Univ. of Western Australia (Australia); Brendan F. Kennedy, The Univ. of Western Australia (Australia), Harry Perkins Institute of Medical Research (Australia), Nicolaus Copernicus Univ. (Poland)
10 April 2024 • 08:30 - 09:00 CEST | Etoile B, Niveau/Level 1
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Optical coherence elastography (OCE) is an imaging technique capable of mapping mechanical properties (such as elasticity) in 3-D and is emerging as a valuable tool in the study and potential intraoperative diagnosis of breast cancer due to mechanical contrast between healthy and malignant tissue. While the correlation between elevated elasticity in OCE and breast cancers has been well established, these studies have primarily focused on binary classifications of tissue as either malignant or benign, ignoring much of the heterogeneity present in breast tissue. In this work, we present a detailed assessment of the microstructures present in human breast tissue images acquired with OCE, identifying regions of interest that corresponded to invasive carcinomas, in situ carcinomas and benign tissue types. We also describe the unique morphological patterns present in each tissue type and provide a framework for the interpretation of breast cancer images acquired with OCE.
13006-34
Author(s): Konstantine Cheishvili, Technische Univ. Delft (Netherlands)
10 April 2024 • 09:00 - 09:20 CEST | Etoile B, Niveau/Level 1
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We quantify the precision and bias of dynamic light scattering optical coherence tomography (DLS-OCT) measurements of the diffusion coefficient and flow speed for first and second-order normalized autocovariance functions. For both diffusion and flow the measurement precision and accuracy are severely limited by correlations between the errors in the normalized autocovariance function. We demonstrate a method of mixing statistically independent normalized autocovariance functions at every time delay for removing these correlations. The mixing method reduces the uncertainty in the obtained parameters by a factor of two but has no effect on the standard error of the mean. We find that the precision in DLS-OCT is identical for different averaging techniques, but that the lowest bias is obtained by averaging the measured correlation functions before fitting the model parameters. With our correlation mixing method it is possible to quantify the precision in DLS-OCT and verify whether the Cramer-Rao bound is reached.
13006-35
Author(s): Junaid Ahmad, Niraj K. Soni, Dmitry G. Revin, Stephen J. Matcher, The Univ. of Sheffield (United Kingdom)
10 April 2024 • 09:20 - 09:40 CEST | Etoile B, Niveau/Level 1
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Atopic dermatitis (or eczema) is a common inflammatory skin condition treated mainly with steroid creams, which can lead to skin thinning. Although expensive, new drugs offer similar effectiveness with fewer side effects, necessitating a long-term skin impact assessment. Optical coherence tomography (OCT) offers non-invasive skin examination, but its popular 1300 nm wavelength limits imaging depth to about 1mm due to scattering. Shifting to 1600 nm can improve penetration depth due to reduced scattering. We aim to measure this improvement by building a dual band 1300 nm / 1600 nm OCT system, presenting initial skin imaging results with this system.
13006-36
Author(s): Rui Yuan, Dmitry G. Revin, Robert A. Byers, Stephen J. Matcher, The Univ. of Sheffield (United Kingdom)
10 April 2024 • 09:40 - 10:00 CEST | Etoile B, Niveau/Level 1
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Atopic dermatitis (AD) often induces vasodilation, potentially impacting the velocity of blood flow within capillaries and vessels. This study aims to quantify these changes in blood flow velocities. We engineered a flow phantom infused with milk to simulate blood flow, utilizing a 1.67 MHz 1310 nm Fourier-domain mode-locked (FDML) OCT system for rapid acquisition and short-interscan imaging. Based on variable interscan time (VISTA) processing [1], we observed a correlation between the calculated decorrelation coefficients and the predetermined flow velocities, spanning a range from 0.16 mm/s to 30 mm/s. These findings enable us to explore our clinical hypotheses with in vivo tests. [1] Hwang, Yunchan, et al. "Retinal blood flow speed quantification at the capillary level using temporal autocorrelation fitting OCTA." Biomedical Optics Express 14.6 (2023): 2658-2677.
13006-37
Author(s): Yue Zhu, Zhenyan Guo, Nanjing Univ. of Science and Technology (China)
10 April 2024 • 10:00 - 10:20 CEST | Etoile B, Niveau/Level 1
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FF-OCT (Full-field optical coherence tomography) is a high-resolution optical sectioning imaging technique for subcellular-scale biological tissues. This work aims to generate new interpretations of cellular and tissue pathophysiology by fitting a linear quantitative relationship between image contrast and scattering properties of biological tissues through FF-OCT volumetric signals. First, the study measures the best-fit curve of FF-OCT spectra and fractal dimension by the classical Kolmogorov model. The fractal dimension of each layer in the volume converges within a range. Measuring the contrast change with the fractal dimension of each layer in the deeper regions can compensate for the depth-resolved aberrations. Fresh ex vivo mouse organs were used as the sample. Results show the FF-OCT images of fresh mouse liver tissue by fractal correction. It enhanced the image contrast in cross-sections in FF-OCT imaging and possibly indicated the distribution of tissues with different properties in axial and lateral directions.
Session 8: Optical Coherence Tomography II
10 April 2024 • 10:40 - 12:10 CEST | Etoile B, Niveau/Level 1
Session Chair: Rowan W. Sanderson, The Univ. of Western Australia (Australia)
13006-38
Author(s): Krzysztof A. Maliszewski, Varvara Vetrova, Univ. of Canterbury (New Zealand); Sylwia M. Kolenderska, Nicolaus Copernicus Univ. (Poland), Univ. of Canterbury (New Zealand)
10 April 2024 • 10:40 - 11:10 CEST | Etoile B, Niveau/Level 1
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We present two neural networks: one capable of producing A-scans with only the second-order nonlinearities removed and another for creating A-scans with only the third-order nonlinearities removed. A spectrum free of nonlinearity (second or third order) can additionally be recovered using a third neural network, enabling the application of the nonlinearity-removing networks in a sequence. Our approach allows for either independent switching off of each order or the simultaneous removal of all orders, offering a tool for analysing the effects of each nonlinearity order individually or simply for performing all-depth, blind OCT data linearisation.
13006-39
Author(s): Nima Tabatabaei, Shiva Sabour, Sarah Houshangi-Tabrizi, Christiane Zoidl, Georg R. Zoidl, York Univ. (Canada)
10 April 2024 • 11:10 - 11:30 CEST | Etoile B, Niveau/Level 1
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This study investigates the relationship between Pannexin 1 and refractive errors using a custom-made 1310nm OCT system, capable of imaging the whole eye. Prior research reported vision-related issues in Panx1 mutants but lacked insights into underlying mechanisms. Obtained OCT results establish a direct link between Pannexin1 (Pannexin 1a, Pannexin 1b, and Panx1DKO) and eye structure alterations, primarily manifesting as axial length elongation and negative RRE values, indicating myopia. Additionally, whole eye OCT imaging identifies several category of defects in the lens epithelium and aphakia. This research underscores Pannexin1's significance in visual system development and refractive errors, further emphasizing the diagnostic potential of optical coherence tomography in ophthalmology. Leveraging zebrafish as a model organism offers a unique opportunity to investigate genetic and cellular mechanisms influencing eye health, advancing our understanding of genetic factors and paving the way for further research and potential therapeutic interventions in the field of ophthalmology.
13006-40
Author(s): Bin Yang, Jiawei Sun, Nektarios Koukourakis, Jürgen W. Czarske, TU Dresden (Germany)
10 April 2024 • 11:30 - 11:50 CEST | Etoile B, Niveau/Level 1
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Optical diffraction tomography (ODT) is a powerful 3D imaging technique with immense potential in fields like cancer diagnosis and drug treatment. However, traditional ODT systems face limitations like the "missing cone" problem, affecting 3D resolution and cancer classification. To address this, fiber-optic dual-beam technology employs controlled laser beams for stable cell rotation, improving tomographic imaging. This improvement is further enhanced by a novel tomographic workflow that incorporates optical flow and deep learning, replacing manual interventions with automated processes. This novel method is validated by reconstructing 3D images of simulated cell phantoms, HL60 human cancer cells, and artificial cell phantoms. Its adaptability extends to diverse imaging techniques, promising advancements in cell biology, innovative therapeutics, and enhanced early-stage cancer diagnostics.
13006-41
Author(s): Arsham Hamidi, Alvaro Gonzalez-Jimenez, Alexander A. Navarini, Philippe C. Cattin, Ferda Canbaz, Univ. Basel (Switzerland)
10 April 2024 • 11:50 - 12:10 CEST | Etoile B, Niveau/Level 1
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Feedback systems are crucial for a safe and accurate laser surgery. Optical coherence tomography (OCT) offers valuable feedback, including visual feedback and tissue differentiation. Detecting tissue type is important to avoid harming adjacent critical tissue, though water and blood in surgical settings may affect OCT’s precision. To address this, we integrated OCT and diffuse reflectance spectroscopy (DRS) for enhanced accuracy.This study employs a 1300 nm swept-source OCT with a 2x2 fiber coupler and double-clad fiber. DRS illumination is integrated, and data is collected using a double-clad fiber, analyzed by a spectrometer. The system’s performance is evaluated on various tissue types.
Session 9: Optical Coherence Tomography III
10 April 2024 • 13:10 - 15:10 CEST | Etoile B, Niveau/Level 1
Session Chair: Gianni Nteroli, Univ. of Kent (United Kingdom)
13006-42
Author(s): Gianni Nteroli, Univ. of Kent (United Kingdom); Manoj Dasa, NKT Photonics A/S (Denmark); Giulia Messa, Institut du Cerveau et de la Moelle Épinière (France); Stella Koutsikou, Univ. of Kent (United Kingdom); Magalie M. Bondu, Peter M. Moselund, NKT Photonics A/S (Denmark); Christos Markos, Technical Univ. of Denmark (Denmark); Adrian G. H. Podoleanu, Univ. of Kent (United Kingdom); Ole Bang, Technical Univ. of Denmark (Denmark); Adrian Bradu, Univ. of Kent (United Kingdom)
10 April 2024 • 13:10 - 13:40 CEST | Etoile B, Niveau/Level 1
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A multimodality visible to near-infrared multi-spectral optoacoustic microscopy (OAM) and optical coherence tomography (OCT) instrument is presented. The OAM channel was used for in-vivo imaging of five endogenous contrast agents in living tadpoles (Xenopus laevis), namely melanin, haemoglobin, collagen, glucose, and lipids. To accurately map absorbers, we developed a novel technique based on the assumption that each voxel in the 3D OAM image has only one chromophore contributing to the optoacoustic signal.
13006-43
Author(s): Freja Høier, Gavrielle R. Untracht, Technical Univ. of Denmark (Denmark); Amanda Øster Andersen, Rigshospitalet (Denmark); Karina Stræde, Univ. of Copenhagen (Denmark); Andreas Kjær, Rigshospitalet (Denmark), Univ. of Copenhagen (Denmark); Peter E. Andersen, Technical Univ. of Denmark (Denmark)
10 April 2024 • 13:40 - 14:00 CEST | Etoile B, Niveau/Level 1
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Sample handling is an important consideration when aiming for replicating in vivo conditions ex vivo for the sake of validating imaging protocols and identifying biomarkers of disease. We tested five different handling methods: Snap frozen in isopentane, directly frozen at -80 °C, slowly frozen in a cryobox with and without cryopreservation media, and formalin fixed. The samples were imaged using SD-OCT (1300nm) for qualitative and quantitative validation based on morphological and optical properties. All handling methods were compared in relation to representative histological image (HE staining), fresh tissue samples as well as each other. The results indicate a significant difference in the attenuation coefficient as well as morphological differences between the five different methods and support the hypothesis that proper sample handling is crucial for obtaining translatable results.
13006-44
Author(s): Mengqiu Duan, Dmitry G. Revin, Stephen J. Matcher, The Univ. of Sheffield (United Kingdom)
10 April 2024 • 14:00 - 14:20 CEST | Etoile B, Niveau/Level 1
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Atopic dermatitis is believed to be driven by abnormal skin barrier properties such as thickness and integrity of stratum corneum. As new generations of treatments for atopic dermatitis are under development, new optical imaging tools which enable to visualise the stratum corneum are thus in demand. Conventional optical coherence tomography (OCT) systems can readily image the epidermis and dermis but lacks the depth resolution to image stratum corneum, except at palmar skin sites. We report a Fourier domain visible light OCT system with a depth resolution of less than 1μm, and results on the stratum corneum thickness from imaging the hands of 11 healthy human subjects. We also investigated the effect of water on the stratum corneum properties.
13006-45
Author(s): Shau Poh Chong, Peter Török, Singapore Ctr. for Environmental Life Sciences Engineering (Singapore)
10 April 2024 • 14:20 - 14:50 CEST | Etoile B, Niveau/Level 1
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Hyperspectral OCT imaging of retina within the visible-near-infrared (VIS-NIR) wavelengths could further improve the achievable axial resolution and harness the spectroscopic information as useful biomarker for retinal diseases. Previous efforts to develop OCT covering both visible and near-infrared wavelengths were hampered by the challenges in designing broadband spectrometer with adequate performance. Here, we described a design and implementation of a broadband spectrometer that could achieve spectral range of more than 450 nm with central wavelength around 680 nm. With it, we further demonstrate in vivo maging of mouse retina using a fiber-based OCT system.
13006-46
Author(s): Pooja Gupta, CSIR - Central Scientific Instruments Organisation (India); Manish Rohilla, Postgraduate Institute of Medical Education & Research, Chandigarh (India); Samir K. Mondal, CSIR - Central Scientific Instruments Organisation (India)
10 April 2024 • 14:50 - 15:10 CEST | Etoile B, Niveau/Level 1
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In this work, the negative axicon probe is employed in an optical coherence phase microscopy (OCPM) system. The phase retrieval is done using conventional Hilbert transform for imaging and the Fresnel relation is used for refractive index measurement. In which, the reflected powers from air–glass and sample–glass interfaces are estimated using a fast-Fourier transform. Our OCPM system provides a phase sensitivity of ∼0.28 mrad and optical path length sensitivity of ∼23pm in air. This system is explored for phase imaging and refractive index measurement of the human male Leydig cells. A typical dimension of a Leydig cell from our system is ~19μm whereas the mean refractive index across a cell is found to be 1.3535. This system has potential to produce the phase imaging along with refractive index measurement of human male Leydig cell.
Session 10: Fluorescence Imaging
10 April 2024 • 15:30 - 17:40 CEST | Etoile B, Niveau/Level 1
Session Chair: Dmitry D. Postnov, Aarhus Univ. (Denmark)
Session 10a runs concurrently with Session 10b (Advanced Imaging & Spectroscopy IV)
13006-48
Author(s): Franz-Josef Schmitt, Amna Shah Mehmood, Christian Tüting, Martin-Luther-Univ. Halle-Wittenberg (Germany); Hoang Trong Phan, Leibniz-Institut für Neue Materialien gGmbH (Germany); Fabian Rieder, Farzin Ghane Golmohamadi, Jan Laufer, Martin-Luther-Univ. Halle-Wittenberg (Germany)
10 April 2024 • 15:30 - 15:50 CEST | Etoile B, Niveau/Level 1
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This study analyses the pH-dependent time resolved fluorescence of mCardinal and mNeptune, two red-shifted fluorescent proteins with applications in biomedical imaging. We utilized molecular dynamics (MD) simulations to illuminate the influence of water molecules on the proteins´ photophysical properties. In mCardinal, the average fluorescence lifetime markedly rises from 0.95 ns at pH 7.0 to 1.25 ns at pH 5.5. Conversely, mNeptune exhibits a constant fluorescence lifetime, showing no pH sensitivity. Through Decay-Associated Spectra and MD simulations, we correlated mCardinal’s pH-induced lifetime changes with its molecular properties. Despite both proteins being equally stabilized by hydrogen bonds, mCardinal’s chromophore formed more water contacts than mNeptune’s. Additionally, the chromophore’s interactions with specific amino acids varied between the two proteins, suggesting distinct differences in the excited state proton transfer as a crucial mechanism for pH sensitivity.
13006-49
Author(s): Uwe Ortmann, Max Tillmann, Felix Koberling, Tino Roehlicke, Michael Wahl, Torsten Langer, Mario Gerecke, PicoQuant GmbH (Germany); Ivan Michel Antolovic, Pi Imaging Technology SA (Switzerland); Rainer Erdmann, PicoQuant GmbH (Germany)
10 April 2024 • 15:50 - 16:10 CEST | Etoile B, Niveau/Level 1
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Confocal microscopy is an essential tool in many academic disciplines due to its intrinsic sectioning capability. The combination with time-resolved single photon detectors and time-correlated single photon counting (TCSPC) devices has established it as the leading platform for time resolved investigation methods such as fluorescence lifetime imaging (FLIM). Recently, high-performance SPAD-arrays featuring few tens of pixels have become available. In this work we present the two central hardware building blocks: PicoQuant’s latest multi-channel TCSPC device and a cooled high-performance 23-pixel SPAD-array developed jointly with Pi Imaging Technologies. We discuss how these open up new possibilities in time-resolved confocal microscopy.
13006-50
Author(s): Sassi Ben Aziza, Patrick J. Merken, Luiz Carlos Paiva Gouveia, Rosa Maria Vinella, Raf Schoofs, Dieter Croux, Vincent Vervenne, Xenics NV (Belgium)
10 April 2024 • 16:10 - 16:30 CEST | Etoile B, Niveau/Level 1
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Spectroscopy is a key scientific technique to study the interaction of matter and electromagnetic radiation namely light. To make it possible, joint efforts are required where the integrated-circuit (IC) plays a major role. With today’s advances in CMOS technologies, increasing high-sensitivity and high-dynamic range becomes essential to cope with increasing demands of today’s applications. This paper proposes an InGaAs back-illuminated line-scan array of pixels adapted for new generations of spectroscopy imagers. It allows an effective 40-kHz frame-rate and 84dB dynamic range measured at low-gain for one frame and consumes only 16mA making it highly compatible with portable applications. The sensor proved a wide spectral range (0.9um to 2.1um) hence enabling application in the near infrared and short-wave domains.
13006-51
Author(s): Parul Singh, Stuti Upadhyay, Hemant Singh, Aniruddha Dan, Prasanna Kumari B., Mukesh Dhanka, Jhuma Saha, Indian Institute of Technology Gandhinagar (India)
10 April 2024 • 16:30 - 16:50 CEST | Etoile B, Niveau/Level 1
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This work focuses on a toxic-free approach to produce carbon nanoparticles (CNPs) using a single organic solvent and the leaf extracts of Murraya Koeniggi and targeting some biomedical applications. Characterization results shows the particle size to be in the range of 20-25 nm with a maximum height of 1.92 nm. The optical characterization confirmed the bright red fluorescence emission in the near infrared region, at 663 nm. Further, this work shows the excellent capability of these green synthesis-derived CNPs to scavenge free radicals via antioxidant assay. The antibacterial property was also investigated against E. coli and S. aureus bacteria, which yielded promising results. Due to the excellent optical properties of CNPs with good stability and less energy consumption, this article also sheds light on the future scope of these in targeting applications such as bio-imaging, therapy processes, drug delivery, and many more.
13006-52
Author(s): Gyana Ranjan Sahoo, Cornell Univ. (United States); Amar Nath Sah, Sikha Ahirwar, Indian Institute of Technology Kanpur (India); Kiran Pandey, Ganesh Shankar Vidyarthi Memorial Medical College (India); Madhur Srivastava, Cornell Univ. (United States); Asima Pradhan, Indian Institute of Technology Kanpur (India)
10 April 2024 • 16:50 - 17:10 CEST | Etoile B, Niveau/Level 1
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A wavelet-transform based denoising and random forest (RF) classification algorithm is utilized to analyze intrinsic fluorescence signals that can detect different grades (CIN-I, CIN-II and CIN-III) of cervical pre-cancer. Recorded fluorescence data are decomposed into corresponding wavelet components applying discrete wavelet transform that employs “coif3” mother wavelet function. The wavelet components are thresholded and the denoised data is reconstructed. A 3-fold cross validation is used for the training of RF model. The calculated Receiver Operating Curves (ROC) of all grades exhibit lower false negatives and higher true positives and true negatives, which indicates good classification.
13006-53
Author(s): Sebastian Kruss, Ruhr-Univ. Bochum (Germany)
10 April 2024 • 17:10 - 17:40 CEST | Etoile B, Niveau/Level 1
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Infectious diseases are a major cause of human mortality and have also a huge impact on agriculture. Optimal methods to detect them would be non-invasive and without extensive sample-taking/processing. We developed a set of near infrared (NIR) fluorescent nanosensors and used them for remote fingerprinting of clinically important bacteria/viruses and to detect pathogen responses in plants [1,2,3]. The nanosensors are based on single-walled carbon nanotubes (SWCNTs) that fluoresce in the NIR optical tissue transparency window. To identify bacteria relevant for humans they were chemically tailored to detect released metabolites as well as specific virulence factors (lipopolysaccharides, siderophores, DNases, proteases) and integrated into functional hydrogel arrays with different sensors. These hydrogels are able to distinguish important bacteria (Staphylococcus aureus, Escherichia coli, …) by NIR imaging. Similar sensors allowed us to visualize the chemical defense of plants in response to pathogens and to detect the corona virus. In summary, such nanosensors in combination with NIR imaging concepts demonstrate huge potential for precise monitoring of pathogens.
Session 11: Advanced Imaging and Spectroscopy II
10 April 2024 • 15:30 - 17:30 CEST | Etoile B, Niveau/Level 1
Session Chair: Dror Fixler, Bar-Ilan Univ. (Israel)
Session 10b runs concurrently with Session 10a (Fluorescence Imaging)
13006-106
Author(s): Dror Fixler, Bar-Ilan Univ. (Israel)
10 April 2024 • 15:30 - 15:50 CEST | Etoile B, Niveau/Level 1
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Various biological surfaces are known to be covered by elaborated micro- and nano-structures, serving a number of functions (e.g. anti-reflective, structural coloration, anti-fouling, pro- or anti-adhesive, etc.) and inspiring numerous industrial applications. Recent years have witnessed a remarkable boost in research in this field. To a large extent, this boost owes to the increasing interdisciplinary of approaches being applied to the study of structured biosurfaces. Sciences as different as classical zoology and botany are inseminated with the advances in genetics and molecular biology; biologists collaborate more and more with nanotechnologists, materials scientists and engineers – all these contribute to the widening of the horizons of research on micro- and nano-structured biological surfaces, and to biomimetic and bioengineering applications of these surfaces in industry. We aim at ‘riding the wave’ of these developments with our proposal. In our talk I will present the main goal of the COST Action “Understanding interaction light – biological surfaces: possibility for new electronic materials and devices”.
13006-107
Author(s): Virgil-Florin Duma, Univ. "Aurel Vlaicu" din Arad (Romania); Cosmin G. Sinescu, Univ. de Medicina si Farmacie "Victor Babes" din Timisoara (Romania); Adrian Bradu, Univ. of Kent (United Kingdom); Adrian G. H. Podoleanu, Univ. of Kent (Romania)
10 April 2024 • 15:50 - 16:10 CEST | Etoile B, Niveau/Level 1
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We present a investigations of dental ceramics using in-house developed swept source (SS) optical coherence tomography (OCT) systems. The issue is related to the loss of calibration of ovens utilized for the fabrication of dental crowns. In the first study [http://dx.doi.org/10.3390/app7060552], metal-ceramics crowns were manufactured, in five study groups, with five maximum sintering temperatures: a normal one, two lower levels and two upper levels (up to +50ºC with regard to normal). OCT B- and C-scans were obtained, and qualitative rules-of-thumb were extracted to assess the oven temperature level by observing ceramic grains bellow the level of the tooth-shaped crowns. The second study [https://doi.org/10.3390/ma12060947] moved to a quantitative assessment of the calibration loss of the ovens. A second type of material (for all-ceramic crowns) was considered, and three levels of its specific temperature were tested. For both ceramics reflectivity curves were obtained from OCT C-scans. These analyses demonstrated that there is (only) one parameter consistent with the shift of the maximum temperature in the oven: the ratio of the maximum and minimum (filtered) reflectivity.
13006-108
Author(s): Faraz Rahimpouresfahani, Pouya Rezai, Nima Tabatabaei, York Univ. (Canada)
10 April 2024 • 16:10 - 16:30 CEST | Etoile B, Niveau/Level 1
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This paper introduces an optimized and affordable optofluidic add-on device for continuous and rapid light sheet imaging of C. elegans. Platform utilizes an optofluidic chip with integrated PDMS microlens to generate a light-sheet and rapidly images worms flowing in a microchannel via a regular microscope. Results on 6-OHDA-exposed and Parkinson’s disease transgenic worms demonstrate the ability of platform in qualitative and quantitative imaging of neuronal degenerations at different ages. The developed affordable platform enables a conventional fluorescent microscope to offer high-content and high-throughput light-sheet images of C. elegans populations which is invaluable to aging, neurodegeneration, and drug discovery studies.
13006-109
Author(s): Igor V. Meglinski, Aston Univ. (United Kingdom); Ivan Lopushenko, Anton Sdobnov, Alexander Bykov, Univ. of Oulu (Finland)
10 April 2024 • 16:30 - 16:50 CEST | Etoile B, Niveau/Level 1
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We explore the application of structured vortex laser beams, or shaped light with orbital angular momentum (OAM), in the diagnosis of cell and cell cultures and the quantitative characterization of biological tissues. To examine the conservation of spin and orbital angular momenta during propagation, we constructed a Mach-Zehnder-like interferometer, equipped with a spatial light modulator (SLM), to generate Laguerre-Gaussian (LG) beams with varying momenta. As the LG beam traverses tissue samples, its interference with a reference plane wave is captured by a camera. Our findings reveal that the OAM of the LG beam is maintained through both normal and cancerous tissue samples, exhibiting a distinct phase shift – or twist of light – which is significantly more sensitive (up to ~1000 times) to changes in the tissue's refractive indices compared to conventional methods. We conclude that leveraging OAM in biomedical diagnosis presents exciting prospects for both groundbreaking biological research and enhanced clinical applications.
13006-110
Author(s): Liangtao Gu, Xinyi Zhu, Liang Chen, Wuwei Ren, ShanghaiTech Univ. (China)
10 April 2024 • 16:50 - 17:10 CEST | Etoile B, Niveau/Level 1
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Miniaturized fluorescence microscope (Miniscope) emerges as a powerful tool for observing neural activities in unrestrained animals. However, its simplified optical design leads to optical distortions and uneven illumination, resulting in poor spatial resolution. Herein, we propose a Continuous Space-Varying Deconvolution (CSV-Deconv) algorithm for image enhancement. By calibrating the point spread function (PSF) through varying illumination and orientation, CSV-Deconv achieves a notable 3.2-fold increase in contrast-to-noise ratio. The algorithm exhibits robust generalizability across diverse Miniscope prototypes, showcasing its efficacy in rectifying spatial distortions. This innovation holds promise for advancing the precision and throughput of neural imaging in freely moving subjects.
13006-111
Author(s): Dror Fixler, Bar-Ilan Univ. (Israel); Michal Katan, Bar-Ilan Univ (Israel); Hamootal Duadi, Bar-Ilan Univ. (Israel)
10 April 2024 • 17:10 - 17:30 CEST | Etoile B, Niveau/Level 1
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In the biomedical field, the reemitted light intensity measured from the tissue depends on both scattering and absorption. In order to separate these variables, we use a physical phenomenon discovered in our lab, called the iso-path length (IPL) point. The IPL point is a specific angle around a cylindrical media, where the light intensity is not affected by the scattering and can serve for self-calibration. For a practical use of this concept, we designed an optic biosensor for measuring physiological parameters such as heart rate, oxygen saturation and respiratory rate, in both ordinary and extreme conditions in a hypoxic chamber.
Hot Topics III
11 April 2024 • 09:00 - 10:35 CEST | Auditorium Schweitzer, Niveau/Level 0
Session Moderator:
Thierry Georges, Oxxius (France)
2024 Symposium Chair

9:00 hrs
Welcome and Opening Remarks
Speaker Introduction
13012-500
Author(s): José Capmany Francoy, Univ. Politècnica de València (Spain)
11 April 2024 • 09:05 - 09:50 CEST | Auditorium Schweitzer, Niveau/Level 0
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Programmable photonic circuits manipulate the flow of light on a chip by electrically controlling a set of tunable analog gates connected by optical waveguides. Light is distributed and spatially rerouted to implement various linear functions by interfering signals along different paths. A general-purpose photonic processor can be built by integrating this flexible hardware in a technology stack comprising an electronic monitoring and controlling layer and a software layer for resource control and programming. This processor can leverage the unique properties of photonics in terms of ultra-high bandwidth, high-speed operation, and low power consumption while operating in a complementary and synergistic way with electronic processors. This talk will review the recent advances in the field and it will also delve into the potential application fields for this technology including, communications, 6G systems, interconnections, switching for data centers and computing.
13006-501
Author(s): Vasilis Ntziachristos, Helmholtz Zentrum München GmbH (Germany)
11 April 2024 • 09:50 - 10:35 CEST | Auditorium Schweitzer, Niveau/Level 0
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Biological discovery is a driving force of biomedical progress. With rapidly advancing technology to collect and analyze information from cells and tissues, we generate biomedical knowledge at rates never before attainable to science. Nevertheless, conversion of this knowledge to patient benefits remains a slow process. To accelerate the process of reaching solutions for healthcare, it would be important to complement this culture of discovery with a culture of problem-solving in healthcare. The talk focuses on recent progress with optical and optoacoustic technologies, as well as computational methods, which open new paths for solutions in biology and medicine. Particular attention is given on the use of these technologies for early detection and monitoring of disease evolution. The talk further shows new classes of imaging systems and sensors for assessing biochemical and pathophysiological parameters of systemic diseases, complement knowledge from –omic analytics and drive integrated solutions for improving healthcare.
Session 12: Advanced Imaging and Spectroscopy III
11 April 2024 • 10:50 - 12:20 CEST | Etoile B, Niveau/Level 1
Session Chair: Sebastian Kruss, Ruhr-Univ. Bochum (Germany)
13006-54
Author(s): Mia Viuf Skøtt, Dmitry D. Postnov, Aarhus Univ. (Denmark)
11 April 2024 • 10:50 - 11:20 CEST | Etoile B, Niveau/Level 1
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We will discuss the theoretical and practical foundations and limitations of high-speed laser speckle contrast imaging and demonstrate some of its applications in neurovascular research. Specifically, we will answer how short exposure time affects the contrast analysis, what camera and the speckle-to-pixel size ratio to use and how to analyse the data. We will present the first results on the pulse wave propagation imaging in the brain of awake and anaesthetised ageing (from 18 to 81 weeks) mice and, finally, discuss the translational perspectives and related image design.
13006-55
Author(s): Sammy Apsel, Vika Tarle, Matan Benyamin, Mirit Hen, Zeev Zalevsky, Nisan Ozana, Bar-Ilan Univ. (Israel)
11 April 2024 • 11:20 - 11:40 CEST | Etoile B, Niveau/Level 1
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A novel method for the detection and analysis of acoustic and blood flow signals is presented and experimentally demonstrated. In this technique, dynamic speckles which arises from sub- μm vibrations are analyzed using laser speckle contrast analysis (LASCA). By using a low-cost RS CMOS image sensor, a high acquisition rate is achieved by implementing row-by-row contrast analysis of the developed speckle field. This approach allows us to achieve an acquisition rate of up to 50 kHz, enabling highly cost-effective analysis of acoustic and blood flow signals.
13006-56
Author(s): Batuhan Dizman, Mustafa Kemal Ruhi, Bogaziçi Üniv. (Turkey)
11 April 2024 • 11:40 - 12:00 CEST | Etoile B, Niveau/Level 1
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Optimal laser energy delivery to target tissue is important to achieve an effective laser-induced hyperthermia (LH) with minimal damage to healthy tissue. Therefore, dosimetry and real-time measurement of temperature play key roles in a successful and safe LH application. In this study, we developed a temperature-controlled 808 nm diode-laser system for LH. The system was tested on phantoms and ex vivo tissues by validating the surface temperatures with a thermal camera. According to the results, the temperature-controlled 808 nm diode-laser system could maintain the surface temperature of the irradiated samples at target values.
13006-57
Author(s): Priya Krishnamurthy, Sujatha N. Unni, Indian Institute of Technology Madras (India); Krupakar Parthasarathy, Sudha Narayani Rao, Sathyabama Institute of Science and Technology (India)
11 April 2024 • 12:00 - 12:20 CEST | Etoile B, Niveau/Level 1
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Biofilms are heterogeneous structures that are formed through a series of steps. It is essential to understand the stage of biofilm formation for several biomedical applications, such as assessing the coating efficacy for hard tissue implants, devising treatment strategies to inhibit biofilm formation in thin tissues, and building biocompatible diagnostic devices. The biofilm formation on different solid substrates such as glass and polypropylene is also studied. The formation of biofilm through different stages is evaluated through parameters extracted from Laser speckle imaging and validated through optical microscopy techniques. These parameters capture the concentration and orientation changes of bacteria through the different stages of biofilm.
Session 13: Advanced Imaging and Spectroscopy IV
11 April 2024 • 13:20 - 15:00 CEST | Etoile B, Niveau/Level 1
Session Chair: Artjoms Suponenkovs, Riga Technical Univ. (Latvia)
13006-58
Author(s): Daniele Pirone, Istituto di Scienze Applicate e Sistemi Intelligenti "Eduardo Caianiello", Consiglio Nazionale delle Ricerche (Italy); Annalaura Montella, CEINGE-Biotecnologie Avanzate (Italy); Beatrice Cavina, Univ. degli Studi di Bologna (Italy); Giusy Giugliano, Michela Schiavo, Istituto di Scienze Applicate e Sistemi Intelligenti "Eduardo Caianiello", Consiglio Nazionale delle Ricerche (Italy); Martina Mugnano, Univ. degli Studi di Napoli Federico II (Italy); Vincenza Cerbone, Giulia Scalia, CEINGE-Biotecnologie Avanzate (Italy); Anna Maria Porcelli, Giuseppe Gasparre, Ivana Kurelac, Univ. degli Studi di Bologna (Italy); Vittorio Bianco, Lisa Miccio, Istituto di Scienze Applicate e Sistemi Intelligenti "Eduardo Caianiello", Consiglio Nazionale delle Ricerche (Italy); Mario Capasso, Achille Iolascon, Pier Luca Maffettone, Univ. degli Studi di Napoli Federico II (Italy); Pasquale Memmolo, Pietro Ferraro, Istituto di Scienze Applicate e Sistemi Intelligenti "Eduardo Caianiello", Consiglio Nazionale delle Ricerche (Italy)
11 April 2024 • 13:20 - 13:40 CEST | Etoile B, Niveau/Level 1
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Liquid biopsy is an emerging and promising biomedical tool that aims to the early cancer diagnosis and the definition of personalized therapies in non-invasive and cost-effective way. Recently, tomographic phase imaging flow cytometry (TPIFC) has been developed as a technique for the reconstruction of the 3D volumetric distribution of the refractive indices (RIs) of single cells flowing along a microfluidic channel. Here we investigate the possibility of exploiting the 3D dataset of single cells recorded by TPIFC to feed a machine learning model, in order to recognize tumor cells with respect to a background of monocytes, which are the most similar cells among the WBCs in terms of morphology. Reported results aim to emulate a real scenario for the label-free liquid biopsy based on the machine learning-powered identification of circulating tumor cells within a blood sample imaged by the TPIFC system.
13006-59
Author(s): Roberto Barreiro Marcos, Frank Sanabria-Macias, Arquimea Research Ctr. (Spain); Pedro Martin-Mateos, Univ. Carlos III de Madrid (Spain); Julio E. Posada-Roman, Arquimea Research Ctr. (Spain); Jose Luis Gonzalez-Mora, Univ. de La Laguna (Spain); Cristina de Dios, Arquimea Research Ctr. (Spain), Univ. Carlos III de Madrid (Spain)
11 April 2024 • 13:40 - 14:00 CEST | Etoile B, Niveau/Level 1
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This paper introduces and validates a novel dual-comb functional Near-Infrared Spectroscopy (DC-fNIRS) technique, marking a significant advancement in the field of Near-Infrared Spectroscopy. DC-fNIRS distinguishes itself by its ability to accurately measure the diffuse time of flight (DTOF) within dispersive media in transmission and, for the first time, in backscattering configurations. The study demonstrates the technique's capability in spatially localizing perturbations within turbid media, showcasing its potential as a cost-effective alternative to traditional methods like functional Magnetic Resonance Imaging (fMRI). Employing a novel experimental setup involving dual Optical Frequency Combs generated from a single continuous wave laser source, the study captures intricate details of DTOF. This methodology allows for a comprehensive analysis of DTOF temporal evolution, enhancing our understanding of light propagation in complex media. The results obtained not only validate the effectiveness of the DC-fNIRS technique but also open new avenues for its application in non-invasive monitoring of brain activity and other critical biological processes.
13006-60
Author(s): Brahim Bessif, Mandana Jalali, Daniel Erni, Univ. Duisburg-Essen (Germany)
11 April 2024 • 14:00 - 14:20 CEST | Etoile B, Niveau/Level 1
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Our study investigates the geometric impact of coupling optical fibers to whispering gallery mode (WGM) microcavities for enhanced sensing of the composition of various exosomes. Employing 2D silica fiber structures, our focus includes a comparative analysis of a polystyrene (PS) coated and non-coated silica disk in aqueous medium. An innovative correlation between coupling distance and sensing quality emerges, with the optimal range identified as 0.25 µm to 0.55 µm, showcasing remarkably high-quality factors exceeding 104 and signifying substantial potential for improved sensing capabilities.
13006-61
Author(s): Mona Shahsavari, Arjan Wiskerke, Mendel Engelaer, Ton G. van Leeuwen, Edwin van der Pol, Amsterdam UMC (Netherlands)
11 April 2024 • 14:20 - 14:40 CEST | Etoile B, Niveau/Level 1
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Flow cytometry (FCM) quantifies sub-µm particles in body fluids as biomarkers. Typical FCM electronics detect particles based on a light scattering signal exceeding a threshold. When the sample dilution is reduced to enhance statistics, simultaneous illumination, and false detections occur. To enable measurements at higher concentrations, a signal processing algorithm was developed. The algorithm was evaluated by measuring dilution series of 150 nm and a mixture (150, 200, and 300 nm) of polystyrene beads. FCM electronics reliably measured up to 6.7×E+7 〖ml〗^(-1) for 150 nm beads and 1.3×E+8 〖ml〗^(-1) for the mixture, while the algorithm achieved concentrations up to 1.5×E+9 〖(ml〗^(-1)), enabling 10-fold faster concentration measurements.
13006-62
Author(s): Van Phuc Nguyen, Univ. of Michigan Kellogg Eye Ctr. (United States); Xueding Wang, Univ. of Michigan (United States); Yannis M. Paulus, Univ. of Michigan Kellogg Eye Ctr. (United States)
11 April 2024 • 14:40 - 15:00 CEST | Etoile B, Niveau/Level 1
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Stem cell therapy holds tremendous potential for treating a wide range of currently incurable diseases, including retinal degenerations and geographic atrophy from macular degeneration. This study aimed to develop a non-invasive multimodality imaging system to track stem cells after transplantation in the subretinal space. Human-induced pluripotent stem cells differentiated to retinal pigment epithelium (hiPSC-RPE) cells were labeled with ultraminiaturized gold nanochains. The labeled hiPSC-RPE cells were then injected into 21 rabbits at 4 days after laser-induced photocoagulation damage to the RPE. Color fundus photography, photoacoustic microscopy, optical coherence tomography and fluorescent imaging were used to monitor the rabbit retina before and after the transplantation. The migration pattern and viability of the cells were monitored up to 6 months with a 37-fold increase in 650nm PAM signal. This approach allowed for the longitudinal evaluation of location of the transplanted stem cells, providing valuable insights for the advancement of stem cell therapies.
Session 14: Microscopy
11 April 2024 • 15:20 - 17:30 CEST | Etoile B, Niveau/Level 1
Session Chair: Krzysztof A. Maliszewski, Univ. of Canterbury (New Zealand)
13006-63
Author(s): Gregory Baethge, ANSYS France SAS (France); Csilla Timar-Fulep, Ansys UK Ltd. (United Kingdom); Stefan Thoene, ANSYS Germany GmbH (Germany); Shin-Sung Kim, Ansys UK Ltd. (United Kingdom); Charly Meyer, ANSYS France SAS (France)
11 April 2024 • 15:20 - 15:50 CEST | Etoile B, Niveau/Level 1
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Endoscopes are medical inspection devices made of optical fibers and an imaging lens at the tip. Miniaturizing the optical system is primordial to ensure agility and accessibility for clinical applications. Metalens-based fiber optics endoscopes offer a promising alternative to conventional devices to reduce the size while maintaining the image quality. In this work, we present a new multiscale metalens design solution for fiber optic endoscopes, utilizing full-wave electromagnetic simulations and ray-tracing techniques.
13006-64
Author(s): Haobijam Johnson Singh, Anil Atalay Appak, Jani Mäkinen, Sanni Erämies, Erdem Sahin, Teemu Ihalainen, Atanas P. Gotchev, Humeyra Caglayan, Tampere Univ. (Finland)
11 April 2024 • 15:50 - 16:10 CEST | Etoile B, Niveau/Level 1
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Exploiting the best of both the worlds of light field and meta-optics to design and demonstrate a cost-effective and compact light field microscopy for high-speed volumetric imaging with high spatio-temporal resolution.
13006-65
Author(s): Quentin Bécar, Paul C. Montgomery, Amir Nahas, Vincent Maioli, ICube, Univ. de Strasbourg (France)
11 April 2024 • 16:10 - 16:30 CEST | Etoile B, Niveau/Level 1
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Imaging a large number of samples is necessary to improve statistical robustness in biological assays. Using standard multiwell plates is not possible in usual light-sheet microscopes. One solution is to use an Oblique Plane Microscope (OPM), which is a special fluorescence light sheet microscope, with a single objective near to the sample. To avoid aberrations, OPM generally uses water or silicon immersion to match the refractive index of the sample. In this work we present an oil-immersed OPM and experimentally demonstrate the possibility of using a primary objective with a higher numerical aperture.
13006-66
Author(s): Javier Santana-Nunez, Laura Quintana-Quintana, Instituto Univ. de Microelectrónica Aplicada, Univ. de Las Palmas de Gran Canaria (Spain); Himar Fabelo, Instituto Univ. de Microelectrónica Aplicada, Univ. de Las Palmas de Gran Canaria (Spain), Fundación Canaria Instituto de Investigación Sanitaria de Canarias (Spain); Samuel Ortega, Nofima (Norway), Instituto Univ. de Microelectrónica Aplicada, Univ. de Las Palmas de Gran Canaria (Spain); Esther Sauras-Colón, Hospital de Tortosa Verge de la Cinta, Institut Català de la Salut, Institut d'Investigació Sanitària Pere Virgili (Spain), Univ. Rovira i Virgili (Spain); Noèlia Gallardo-Borràs, Univ. Rovira i Virgili (Spain), Hospital de Tortosa Verge de la Cinta, Institut Català de la Salut, Institut d'Investigació Sanitària Pere Virgili (Spain); Daniel Mata-Cano, Hospital de Tortosa Verge de la Cinta, Institut Català de la Salut, Institut d'Investigació Sanitària Pere Virgili (Spain); Carlos López-Pablo, Hospital de Tortosa Verge de la Cinta, Institut Català de la Salut, Institut d'Investigació Sanitària Pere Virgili (Spain), Univ. Rovira i Virgili (Spain); Gustavo M. Callico, Instituto Univ. de Microelectrónica Aplicada, Univ. de Las Palmas de Gran Canaria (Spain)
11 April 2024 • 16:30 - 16:50 CEST | Etoile B, Niveau/Level 1
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Cancer is one of the worldwide leading causes of death. They are diagnosed and graded histologically using biopsies, sliced at 2-5 μm, and stained with Haematoxylin and Eosin (H&E). Hyperspectral (HS) Imaging (HSI) has been arising to improve the spectral information given by RGB (Red-Green-Blue) cameras. This work aims to characterize the influence of tissue thickness on the spectral information. Captures were taken over breast tissue samples sectioned at 2 and 3 μm. They were pre-processed to reduce noise and normalize the data. Then, based on H&E absorption spectra peaks, HS images could be segmented into stroma (eosin-stained basic structures), nucleus (haematoxylin-stained acidic structures), and background (non-stained structures). Concluding, results show that 3 μm slides proportionate a bigger count of cells and a higher spectral contrast than 2 μm slides. However, further research is needed to study other thickness values, including more samples.
13006-105
Author(s): Giuseppe Chirico, Laura Sironi, Davide Panzeri, Univ. degli Studi di Milano-Bicocca (Italy); Claudio Conci, Politecnico di Milano (Italy); Donato Inverso, I.R.C.C.S. Ospedale San Raffaele (Italy); Emanuela Jacchetti, Politecnico di Milano (Italy); Rebeca Martínez Vázquez, Roberto Osellame, Istituto di Fotonica e Nanotecnologie, Consiglio Nazionale delle Ricerche (Italy), Politecnico di Milano (Italy); Maddalena Collini, Univ. degli Studi di Milano-Bicocca (Italy); Giulio Cerullo, Manuela Teresa Raimondi, Politecnico di Milano (Italy)
11 April 2024 • 16:50 - 17:10 CEST | Etoile B, Niveau/Level 1
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Tissue histopathology, reliant on costly and time-consuming hematoxylin and eosin (H&E) staining of thin tissue slices, faces limitations. Label-free non-linear optical microscopy in vivo presents a solution, allowing work on fresh samples. Implantable microstructures prove effective for systematic longitudinal in vivo studies of immunological responses to biomaterials using label-free non-linear optical microscopy. Employing two-photon laser polymerization, we implanted a matrix of 3D lattices in the chorioallantoic membrane of chicken embryos, establishing a 3D reference frame for cell counting. H&E analysis is compared to label-free in vivo non-linear excitation imaging for cell quantification and identifying granulocytes, collagen, and microvessels. Preliminary results in higher animal models demonstrate the transformative potential of this approach, offering an alternative to conventional histopathology for validating biomaterials in in vivo longitudinal studies.
13006-68
Author(s): Gyana Ranjan Sahoo, Cornell Univ. (United States); Jaidip M. Jagtap, Mayo Clinic (United States); Prasanta K. Panigrahi, Indian Institute of Science Education and Research Kolkata (India); Asima Pradhan, Indian Institute of Technology Kanpur (India); Madhur Srivastava, Cornell Univ. (United States)
11 April 2024 • 17:10 - 17:30 CEST | Etoile B, Niveau/Level 1
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A Bayesian statistics-based image segmentation algorithm is applied on unstained tissue section images to distinguish normal and different grades (CIN-I, CIN-II, CIN-III) of cervical pre-cancer. This multi-level thresholding achieves an effective image quantization of the high cell density domain, most affected during the progression of the disease, which yields a precise visualization of the lesions in the epithelial cellular structures, revealing their spatial changes with the progression of the disease. The pixel count ratio of the quantized high cell density region drops below a statistically well-defined threshold, that quantitatively discriminates the different grades. The Receiver Operating Curve (ROC) exhibits good discrimination between normal and different pre-cancer grades.
13006-69
Author(s): Jawaria Maqbool, Syed Talal Hassan, M. Imran Cheema, Lahore Univ. of Management Sciences (Pakistan)
11 April 2024 • 17:30 - 17:50 CEST | Etoile B, Niveau/Level 1
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For multimode fiber imaging, deep learning's capability in handling complex data relationships is undeniable. However, it demands a substantial amount of high-quality data, and it takes 24 hours to measure speckle patterns for 60,000 MNIST digits. Therefore, we turn to conditional generative adversarial networks (CGANs) for data augmentation. Our CGAN model synthesizes data closely resembling experimental speckle patterns for MNIST digits, with an SSIM of 0.9840. With only 16,500 training samples, we generate an additional 38,500 speckle patterns in just 30 minutes. This research highlights the CGAN's potential in transcending data collection limitations for multimode fiber endoscopy and industrial inspection.
Conference Chair
Leibniz-Institut für Photonische Technologien e.V. (Germany)
Conference Chair
Lab. Charles Coulomb (France)
Program Committee
Technical Univ. of Denmark (Denmark)
Program Committee
James M. Brewer
Univ. of Glasgow (United Kingdom)
Program Committee
National Yang-Ming Univ. (Taiwan)
Program Committee
TU Dresden (Germany)
Program Committee
Vrije Univ. Amsterdam (Netherlands)
Program Committee
Univ. of St. Andrews (United Kingdom)
Program Committee
Bar-Ilan Univ. (Israel)
Program Committee
Univ. de Strasbourg (France)
Program Committee
Univ. of Houston (United States)
Program Committee
Hainan Univ. (China)
Program Committee
Leibniz-Institut für Photonische Technologien e.V. (Germany)
Program Committee
Helmholtz Zentrum München GmbH (Germany)
Program Committee
The Univ. of Western Australia (Australia)
Program Committee
Johann Wolfgang Goethe-Univ. Frankfurt am Main (Germany)
Program Committee
Vrije Univ. Brussel (Belgium)
Program Committee
Indian Institute of Science (India)
Program Committee
Ontario Cancer Institute (Canada)
Program Committee
Ctr. for Biomedical Optics and Photonics (Germany)
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