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Spie Press Book

Optical Phantoms: Diffuse and Sub-diffuse Imaging and Spectroscopy Validation
Author(s): Gage J. Greening; Haley M. James; Timothy J. Muldoon
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Book Description

This Spotlight describes the use of optical phantoms as validation tools for probe-based imaging and spectroscopy platforms. Composition of optical phantoms, including scattering and absorbing agents, is discussed in the context of their specific application. Next, requirements for designing optical phantoms for validation of small endoscopic instrumentation is presented including a discussion on the diffuse and sub-diffuse scattering regimes and how this relates to common types of epithelial tissue. This book concludes with a tutorial section for constructing solid phantoms to simulate thin epithelial tissue structure and liquid phantoms to extract tissue optical properties using probe-based reflectance spectroscopy techniques. For these applications, solid phantoms are made using poly(dimethylsiloxane) and liquid phantoms are made using deionized water as the substrate material. The scope of the book is limited to optical phantom design and application and is not intended to describe the physics, instrumentation, or mathematical or computational modeling of the various techniques mentioned in detail.

Book Details

Date Published: 25 November 2015
Pages: 40
ISBN: 9781510600324
Volume: SL09

Table of Contents
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1 Introduction
      1.1 Optical phantoms in probe-based systems
     1.1.1 High-resolution microendoscopy
     1.1.2 Hyperspectral imaging
     1.1.3 Diffuse optical tomography
     1.1.4 Reflectance spectroscopy
     1.1.5 Fluorescence spectroscopy
     1.1.6 Raman spectroscopy

2 Requirements for Small Endoscopic Instrumentation
2.1 Subdiffuse scattering regime
2.2 Geometry and optical properties of epithelial tissue

3 Fabrication of Phantoms for Optical Probe Design
3.1 Solid phantoms as tools for simulating epithelial structure
     3.1.1 Constructing the PDMS-based mix
     3.1.2 Spin coating technique for creation of thin phantoms
3.2 Liquid phantoms as tools for quantifying optical properties
     3.2.1 Building a lookup table using optical calibration phantoms
     3.2.2 Evaluating lookup table using optical validation phantoms

4 Conclusion

5 Acknowledgments

References

Preface

This Spotlight describes the use of optical phantoms as validation tools for probe-based imaging and spectroscopy platforms. Composition of optical phantoms, including scattering and absorbing agents, is discussed in the context of their specific application. Next, requirements for designing optical phantoms for validation of small endoscopic instrumentation is presented including a discussion on the diffuse and sub-diffuse scattering regimes and how this relates to common types of epithelial tissue. This Spotlight concludes with a tutorial section for constructing solid phantoms to simulate thin epithelial tissue structure and liquid phantoms to extract tissue optical properties using probe-based reflectance spectroscopy techniques. For these applications, solid phantoms are made using poly(dimethylsiloxane) and liquid phantoms are made using deionized water as the substrate material. The scope of the chapter is limited to optical phantom design and application and is not intended to describe the physics, instrumentation, or mathematical or computational modeling of the various techniques mentioned in detail.

Gage J. Greening
Haley M. James
Timothy J. Muldoon
November 2015


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